Values CatLog read from this paper
Kinetics and thermodynamics of the protein-ligand interactions in the riboflavin kinase activity of the FAD synthetase from Corynebacterium ammoniagenes
PMID 28779158doi:10.1038/s41598-017-07875-5Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (24 values) from this paper: FAD synthetase (CaFADS), riboflavin kinase (RFK) module from Corynebacterium ammoniagenes.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateno product added; Michaelis-Menten fit | 2.7 | 0.06 | 44 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit | 2.7 | 0.11 | 24 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit | 2.3 | 0.14 | 17 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit | 2.8 | 0.26 | 11 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateATP varied (10–350 µM) at ~5 µM RF; Michaelis-Menten fit | 2.8 | 0.7 | 4.0 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | adenosine 5'-triphosphateATP varied at ~5 µM RF | 2.2 | 0.04 | 54 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, adenosine 5'-triphosphate |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied (0.5–45 µM) at saturating ATP (350 µM) | 7.3 | 0.01 | 730 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM) | 4.7 | 0.005 | 930 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM) | 4.5 | 0.0077 | 580 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM) | 2.3 | 0.0036 | 650 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied (up to 7 µM) at saturating ATP (350 µM) | 2 | 0.0055 | 360 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |
| FAD synthetase (CaFADS), riboflavin kinase (RFK) module | Corynebacterium ammoniagenes | riboflavinRF varied at saturating ATP (350 µM) | 5.3 | 0.0069 | 770 calc. | pH 7, 25 °C | Open record: FAD synthetase (CaFADS), riboflavin kinase (RFK) module, riboflavin |