Values CatLog read from this paper
The trimer interface in the quaternary structure of the bifunctional prokaryotic FAD synthetase from Corynebacterium ammoniagenes
PMID 28341845doi:10.1038/s41598-017-00402-6Open in Browse
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CatLog read 48 records (131 values) from this paper: bifunctional FAD synthetase (CaFADS) from Corynebacterium ammoniagenes.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| bifunctional FAD synthetase (CaFADS)variant D298A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.537 | 0.0266 | 20 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant D298A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0207 | 5 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant D298A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.102 | 0.0012 | 85 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant D298A | Corynebacterium ammoniagenes | riboflavin | 1.307 | 7 × 10⁻⁴ | 152 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant D298E | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.348 | 0.0649 | 5.3 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298E, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant D298E | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0104 | 5.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298E, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant D298E | Corynebacterium ammoniagenes | flavin mononucleotide | 0.055 | 9.5 × 10⁻⁴ | 57.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant D298E, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant E203A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.835 | 0.0892 | 9.3 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E203A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E203A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0108 | 5 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E203A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E203A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.053 | 7 × 10⁻⁴ | 75 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E203A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant E203A | Corynebacterium ammoniagenes | riboflavin | 2.13 | 0.0037 | 577 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E203A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant E301A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.612 | 0.0309 | 20 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E301A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0197 | 5.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E301A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.115 | 4.2 × 10⁻⁴ | 273 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant E301A | Corynebacterium ammoniagenes | riboflavin | 0.883 | 1 × 10⁻⁴ | 170 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant E301K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.998 | 0.0593 | 17 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E301K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0153 | 6.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant E301K | Corynebacterium ammoniagenes | flavin mononucleotide | 0.105 | 8.5 × 10⁻⁴ | 123 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant E301K, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant F206A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 2.32 | 0.121 | 19 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.038 | 1.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.07 | 0.0029 | 24.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant F206A | Corynebacterium ammoniagenes | riboflavin | 3.15 | 0.0032 | 995 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant F206K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 2.5 | 0.14 | 17.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0388 | 2.7 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206K | Corynebacterium ammoniagenes | flavin mononucleotide | 0.103 | 0.0054 | 19.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206K, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant F206K | Corynebacterium ammoniagenes | riboflavin | 2.93 | 0.004 | 740 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206K, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant F206W | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 1.17 | 0.0247 | 47.3 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206W, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206W | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0252 | 3.7 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206W, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant F206W | Corynebacterium ammoniagenes | flavin mononucleotide | 0.077 | 0.0017 | 55 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant F206W, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant K202A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 0.575 | 0.0527 | 11 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant K202A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant K202A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0121 | 3.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant K202A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant K202A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.038 | 0.0029 | 13.3 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant K202A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant K202A | Corynebacterium ammoniagenes | riboflavin | 1.063 | 8 × 10⁻⁴ | 165 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant K202A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant L304A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 2.95 | 0.004 | 738 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant L304A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0347 | 1.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant L304A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.042 | 0.0028 | 15.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant L304A | Corynebacterium ammoniagenes | riboflavin | 4.33 | 0.0033 | 1313 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304A, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant L304K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 2.18 | 0.0048 | 457 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant L304K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0115 | 7.5 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant L304K | Corynebacterium ammoniagenes | flavin mononucleotide | 0.087 | 0.0152 | 5.7 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304K, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant L304K | Corynebacterium ammoniagenes | riboflavin | 9 | 0.0149 | 607 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant L304K, riboflavin |
| bifunctional FAD synthetase (CaFADS)variant V300A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 1.162 | 0.0375 | 31 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant V300A | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0462 | 1.8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300A, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant V300A | Corynebacterium ammoniagenes | flavin mononucleotide | 0.082 | 0.0014 | 58 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300A, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant V300K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateRFK activity (RF + ATP -> FMN + ADP); 0.8 mM MgCl2; 1 min | 3.12 | 0.00725 | 430 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant V300K | Corynebacterium ammoniagenes | adenosine 5'-triphosphateFMNAT activity (FMN -> FAD); 10 mM MgCl2; FMN 0-15 µM | — | 0.0095 | 8 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300K, adenosine 5'-triphosphate |
| bifunctional FAD synthetase (CaFADS)variant V300K | Corynebacterium ammoniagenes | flavin mononucleotide | 0.077 | 0.0083 | 9.2 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300K, flavin mononucleotide |
| bifunctional FAD synthetase (CaFADS)variant V300K | Corynebacterium ammoniagenes | riboflavin | 5.98 | 0.0097 | 613 | pH 7, 25 °C | Open record: bifunctional FAD synthetase (CaFADS) variant V300K, riboflavin |