Values CatLog read from this paper
Tryptophan-47 in the active site of Methylophaga sp. strain SK1 flavin-monooxygenase is important for hydride transfer
PMID 23357278doi:10.1016/j.abb.2013.01.004Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (26 values) from this paper: flavin-containing monooxygenase (FMO) from Methylophaga sp. strain SK1.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| flavin-containing monooxygenase (FMO) | Methylophaga sp. strain SK1 | Methimazole | 2.7 | 0.163 | 16 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO), Methimazole |
| flavin-containing monooxygenase (FMO) | Methylophaga sp. strain SK1 | NADPHcosubstrate trimethylamine (20 μM, saturating) | 7.3 | 0.0041 | 1.8 × 10³ | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO), NADPH |
| flavin-containing monooxygenase (FMO) | Methylophaga sp. strain SK1 | NADPHoxidase activity in the absence of second substrate | 0.08 | — | — | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO), NADPH |
| flavin-containing monooxygenase (FMO) | Methylophaga sp. strain SK1 | Oxygen | 7.7 | 0.019 | 410 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO), Oxygen |
| flavin-containing monooxygenase (FMO) | Methylophaga sp. strain SK1 | Trimethylamine | 6.1 | 0.0026 | 2.3 × 10³ | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO), Trimethylamine |
| flavin-containing monooxygenase (FMO)variant W47F | Methylophaga sp. strain SK1 | Methimazole | 0.67 | 0.037 | 18 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO) variant W47F, Methimazole |
| flavin-containing monooxygenase (FMO)variant W47F | Methylophaga sp. strain SK1 | NADPHcosubstrate trimethylamine (20 μM, saturating) | 0.92 | 0.0043 | 210 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO) variant W47F, NADPH |
| flavin-containing monooxygenase (FMO)variant W47F | Methylophaga sp. strain SK1 | NADPHoxidase activity in the absence of second substrate | 0.17 | — | — | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO) variant W47F, NADPH |
| flavin-containing monooxygenase (FMO)variant W47F | Methylophaga sp. strain SK1 | Oxygen | 2.7 | 0.042 | 60 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO) variant W47F, Oxygen |
| flavin-containing monooxygenase (FMO)variant W47F | Methylophaga sp. strain SK1 | Trimethylamine | 1.25 | 0.0015 | 800 | pH 8.5, 25 °C | Open record: flavin-containing monooxygenase (FMO) variant W47F, Trimethylamine |