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Structure and substrate specificity of the pyrococcal coenzyme A disulfide reductases/polysulfide reductases (CoADR/Psr): implications for S(0)-based respiration and a sulfur-dependent antioxidant…
PMID 23530771doi:10.1021/bi3014399Open in Browse
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CatLog read 11 records (31 values) from this paper: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | 5,5'-dithiobis-2-nitrobenzoic acid | 0.69 | 0.129 | 5.35 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, 5,5'-dithiobis-2-nitrobenzoic acid |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | CoA polysulfide | 6.56 | 0.0949 | 69 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, CoA polysulfide |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | coenzyme A persulfide100 µM NADH fixed (Table 2 footnote a); cosubstrate NADH | 1.11 | 0.0205 | 54 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, coenzyme A persulfide |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | coenzyme A persulfide100 µM NADPH fixed (Table 2 footnote a); cosubstrate NADPH | 1.5 | 0.0562 | 27 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, coenzyme A persulfide |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | glutathione persulfide | 0.35 | — | — | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, glutathione persulfide |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | NADHpersulfide; cosubstrate CoA persulfide | 1.07 | 0.0139 | 77 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, NADH |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | NADHpolysulfide; cosubstrate CoA polysulfide | 8.86 | 0.125 | 79 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, NADH |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | NADPHpersulfide; cosubstrate CoA persulfide | 2.08 | 0.0127 | 160 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, NADPH |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | NADPHpolysulfide; cosubstrate CoA polysulfide | 16.1 | 0.0141 | 1.2 × 10³ | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, NADPH |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | polysulfide | 1.04 | 0.0123 | 85 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, polysulfide |
| coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR | Pyrococcus horikoshii | TNB persulfide | 3.37 | 0.0251 | 135 | pH 8.1, 50 °C | Open record: coenzyme A disulfide reductase/polysulfide reductase (CoADR/Psr), phCoADR, TNB persulfide |