Values CatLog read from this paper

Engineering the synthetic potential of β-lactam synthetase and the importance of catalytic loop dynamics

PMID 23616913doi:10.1039/c2md00305hOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (31 values) from this paper: β-lactam synthetase (βLS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.3.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Open record
β-lactam synthetase (βLS)	—	N2-(2-carboxyethyl)-L-arginine	0.87	0.037	24 calc.	Open record: β-lactam synthetase (βLS), N2-(2-carboxyethyl)-L-arginine
β-lactam synthetase (βLS)	—	N2-[(2R)-2-carboxybutyl]-L-arginine	0.004	—	—	Open record: β-lactam synthetase (βLS), N2-[(2R)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)	—	N2-[(2R)-2-carboxypropyl]-L-arginine	0.032	0.04	0.80 calc.	Open record: β-lactam synthetase (βLS), N2-[(2R)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)	—	N2-[(2S)-2-carboxybutyl]-L-arginine	0.0023	0.06	0.038 calc.	Open record: β-lactam synthetase (βLS), N2-[(2S)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)	—	N2-[(2S)-2-carboxypropyl]-L-arginine	0.023	0.06	0.38 calc.	Open record: β-lactam synthetase (βLS), N2-[(2S)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant H447A	—	N2-(2-carboxyethyl)-L-arginine	0.016	0.9	0.018 calc.	Open record: β-lactam synthetase (βLS) variant H447A, N2-(2-carboxyethyl)-L-arginine
β-lactam synthetase (βLS)variant H447A	—	N2-[(2R)-2-carboxypropyl]-L-arginine	0.003	—	—	Open record: β-lactam synthetase (βLS) variant H447A, N2-[(2R)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant H447A	—	N2-[(2S)-2-carboxybutyl]-L-arginine	0.001	—	—	Open record: β-lactam synthetase (βLS) variant H447A, N2-[(2S)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)variant H447A	—	N2-[(2S)-2-carboxypropyl]-L-arginine	0.013	0.08	0.16 calc.	Open record: β-lactam synthetase (βLS) variant H447A, N2-[(2S)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant V446A	—	N2-(2-carboxyethyl)-L-arginine	0.28	0.36	0.78 calc.	Open record: β-lactam synthetase (βLS) variant V446A, N2-(2-carboxyethyl)-L-arginine
β-lactam synthetase (βLS)variant V446A	—	N2-[(2R)-2-carboxybutyl]-L-arginine	0.004	—	—	Open record: β-lactam synthetase (βLS) variant V446A, N2-[(2R)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)variant V446A	—	N2-[(2R)-2-carboxypropyl]-L-arginine	0.014	0.33	0.042 calc.	Open record: β-lactam synthetase (βLS) variant V446A, N2-[(2R)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant V446A	—	N2-[(2S)-2-carboxybutyl]-L-arginine	0.004	0.04	0.10 calc.	Open record: β-lactam synthetase (βLS) variant V446A, N2-[(2S)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)variant V446A	—	N2-[(2S)-2-carboxypropyl]-L-arginine	0.34	0.46	0.74 calc.	Open record: β-lactam synthetase (βLS) variant V446A, N2-[(2S)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant V446G	—	N2-(2-carboxyethyl)-L-arginine	0.13	0.61	0.21 calc.	Open record: β-lactam synthetase (βLS) variant V446G, N2-(2-carboxyethyl)-L-arginine
β-lactam synthetase (βLS)variant V446G	—	N2-[(2R)-2-carboxypropyl]-L-arginine	0.07	1.9	0.037 calc.	Open record: β-lactam synthetase (βLS) variant V446G, N2-[(2R)-2-carboxypropyl]-L-arginine
β-lactam synthetase (βLS)variant V446G	—	N2-[(2S)-2-carboxybutyl]-L-arginine	0.002	—	—	Open record: β-lactam synthetase (βLS) variant V446G, N2-[(2S)-2-carboxybutyl]-L-arginine
β-lactam synthetase (βLS)variant V446G	—	N2-[(2S)-2-carboxypropyl]-L-arginine	0.13	1.2	0.11 calc.	Open record: β-lactam synthetase (βLS) variant V446G, N2-[(2S)-2-carboxypropyl]-L-arginine