CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutant IDH1 enhances the production of 2-hydroxyglutarate due to its kinetic mechanism

PMID 23731180doi:10.1021/bi400514kOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (76 values) from this paper: IDH1 (isocitrate dehydrogenase 1) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.1.1.42

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
IDH1 (isocitrate dehydrogenase 1)Homo sapienscarbon dioxide (added as sodium bicarbonate)CO2 varied with NADPH fixed at 200 µM and αKG at 0.5 mM2.020.6363.2pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)Homo sapienscarbon dioxide (added as sodium bicarbonate)CO2 varied with NADPH fixed at 50 µM and αKG at 0.5 mM1.570.6662.3pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)Homo sapiensisocitrate8.620.0051.72 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)Homo sapiensNADP+—0.00621.38 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)Homo sapiensNADPHN-OG dead-end2.280.060238pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)Homo sapiensNADPHNAADP+ dead-end2.730.047257.8pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)Homo sapiensα-ketoglutarateαKG varied with NADPH fixed at 200 µM and CO2 at 2.28 mM2.230.13816.2pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)Homo sapiensα-ketoglutarateαKG varied with NADPH fixed at 50 µM and CO2 at 2.28 mM1.4370.3813.8pH 8, 25 °COpen
IDH1 (isocitrate dehydrogenase 1)variant G97DHomo sapiensisocitrate0.9680.2963.3pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97DHomo sapiensNADP+—4.6 × 10⁻⁴2.1 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97DHomo sapiensNADPH0.03972.5 × 10⁻⁴158.7pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97DHomo sapiensα-ketoglutarate0.05420.2180.25pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97NHomo sapiensisocitrate0.1024.6 × 10⁻⁴220pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97NHomo sapiensNADP+—6 × 10⁻⁴170pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97NHomo sapiensNADPH0.03251.1 × 10⁻⁴295pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant G97NHomo sapiensα-ketoglutarate0.0420.2950.15pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensisocitrate0.1286.60.02pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensNADP+—5.5 × 10⁻⁵2.33 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensNADPHNADPH varied with αKG fixed at 1 mM; Table 20.754.3 × 10⁻⁴1.75 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensNADPHNADPH varied with αKG fixed at 1 mM; Table 20.5123 × 10⁻⁴1.7 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensNADPHFigure 6A—4 × 10⁻⁴—pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensα-ketoglutarateαKG varied with NADPH fixed at 50 µM0.7131.080.67pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensα-ketoglutarateαKG varied with NADPH fixed at 0.5 µM0.5321.330.4pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant R132HHomo sapiensα-ketoglutarate0.241.80.13 calc.pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant Y139DHomo sapiensisocitrate0.01180.00244.8pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant Y139DHomo sapiensNADP+—1.1 × 10⁻⁵1.05 × 10³pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant Y139DHomo sapiensNADPH0.01681.6 × 10⁻⁴105.2pH 8Open
IDH1 (isocitrate dehydrogenase 1)variant Y139DHomo sapiensα-ketoglutarate0.01730.07420.23pH 8Open