Values CatLog read from this paper

Activity of ADAM17 (a disintegrin and metalloprotease 17) is regulated by its noncatalytic domains and secondary structure of its substrates

PMID 23779109doi:10.1074/jbc.m113.462267Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 37 records (81 values) from this paper: ADAM17 (TACE), ADAM10, ADAM12.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.24.86

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
ADAM10	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)	0.34	0.012	28	25 °C	Open record: ADAM10, glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM10	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)	0.02	0.002	9	25 °C	Open record: ADAM10, nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM10	—	substrate 5 (TNFα residues 73-82)	0.03	0.01	3.0 calc.	25 °C	Open record: ADAM10, substrate 5 (TNFα residues 73-82)
ADAM10	—	substrate 6 (juxtamembrane, TNFα residues 57-82)	0.06	0.013	5	25 °C	Open record: ADAM10, substrate 6 (juxtamembrane, TNFα residues 57-82)
ADAM10	—	substrate 7 (TNFα residues 72-108)	0.013	0.0043	3.0 calc.	25 °C	Open record: ADAM10, substrate 7 (TNFα residues 72-108)
ADAM10	—	substrate 8 (two α-helix-inducing heptads)	0.03	0.0062	3.9	25 °C	Open record: ADAM10, substrate 8 (two α-helix-inducing heptads)
ADAM10	—	substrate 9 (α-helical, three α-helix-inducing heptads)	0.004	0.0055	0.6	25 °C	Open record: ADAM10, substrate 9 (α-helical, three α-helix-inducing heptads)
ADAM12	—	substrate 6 (juxtamembrane, TNFα residues 57-82)	0.006	0.018	0.3	25 °C	Open record: ADAM12, substrate 6 (juxtamembrane, TNFα residues 57-82)
ADAM12	—	substrate 8 (two α-helix-inducing heptads)	0.003	0.0062	0.5	25 °C	Open record: ADAM12, substrate 8 (two α-helix-inducing heptads)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	—	—	32	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	0.22	—	—	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	—	0.0069	—	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	—	—	36	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	0.36	—	—	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)catalytic domain (CD); residues Arg215-Val477	—	0.01	—	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 1 (TNFα-based fluorogenic peptide)ectodomain (ECD); residues Arg215-Asn671	0.08	0.0032	23	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 1 (TNFα-based fluorogenic peptide)
ADAM17 (TACE)	—	glycosylated substrate 2 (TNFα residues 73-82)ectodomain (ECD); residues Arg215-Asn671	0.42	0.0076	61	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 2 (TNFα residues 73-82)
ADAM17 (TACE)	—	glycosylated substrate 2 (TNFα residues 73-82)catalytic domain (CD); residues Arg215-Val477	0.08	0.068	1.1	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 2 (TNFα residues 73-82)
ADAM17 (TACE)	—	glycosylated substrate 3 (TNFα residues 73-82, bulkier carbohydrate)ectodomain (ECD); residues Arg215-Asn671	0.15	0.022	6.8	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 3 (TNFα residues 73-82, bulkier carbohydrate)
ADAM17 (TACE)	—	glycosylated substrate 3 (TNFα residues 73-82, bulkier carbohydrate)catalytic domain (CD); residues Arg215-Val477	0.06	0.05	1.2	25 °C	Open record: ADAM17 (TACE), glycosylated substrate 3 (TNFα residues 73-82, bulkier carbohydrate)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	—	—	17	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	0.07	—	—	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	—	0.004	—	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	—	—	18	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	0.06	—	—	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)catalytic domain (CD); residues Arg215-Val477	—	0.0034	—	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)ectodomain (ECD); residues Arg215-Asn671	0.1	0.004	19	25 °C	Open record: ADAM17 (TACE), nonglycosylated substrate 4 (TNFα-based fluorogenic peptide, residues 72-81)
ADAM17 (TACE)	—	substrate 5 (TNFα residues 73-82)ectodomain (ECD); residues Arg215-Asn671	0.2	0.0072	28 calc.	25 °C	Open record: ADAM17 (TACE), substrate 5 (TNFα residues 73-82)
ADAM17 (TACE)	—	substrate 5 (TNFα residues 73-82)catalytic domain (CD); residues Arg215-Val477	0.05	0.016	3.1 calc.	25 °C	Open record: ADAM17 (TACE), substrate 5 (TNFα residues 73-82)
ADAM17 (TACE)	—	substrate 6 (juxtamembrane, TNFα residues 57-82)ectodomain (ECD); residues Arg215-Asn671	0.34	0.024	15	25 °C	Open record: ADAM17 (TACE), substrate 6 (juxtamembrane, TNFα residues 57-82)
ADAM17 (TACE)	—	substrate 6 (juxtamembrane, TNFα residues 57-82)catalytic domain (CD); residues Arg215-Val477	0.12	0.02	6.2	25 °C	Open record: ADAM17 (TACE), substrate 6 (juxtamembrane, TNFα residues 57-82)
ADAM17 (TACE)	—	substrate 7 (TNFα residues 72-108)ectodomain (ECD); residues Arg215-Asn671	0.07	0.0033	21 calc.	25 °C	Open record: ADAM17 (TACE), substrate 7 (TNFα residues 72-108)
ADAM17 (TACE)	—	substrate 7 (TNFα residues 72-108)catalytic domain (CD); residues Arg215-Val477	0.03	0.0043	7.0 calc.	25 °C	Open record: ADAM17 (TACE), substrate 7 (TNFα residues 72-108)
ADAM17 (TACE)	—	substrate 8 (two α-helix-inducing heptads)ectodomain (ECD); residues Arg215-Asn671	0.064	0.0075	9	25 °C	Open record: ADAM17 (TACE), substrate 8 (two α-helix-inducing heptads)
ADAM17 (TACE)	—	substrate 8 (two α-helix-inducing heptads)catalytic domain (CD); residues Arg215-Val477	0.07	0.0079	9	25 °C	Open record: ADAM17 (TACE), substrate 8 (two α-helix-inducing heptads)
ADAM17 (TACE)	—	substrate 9 (α-helical, three α-helix-inducing heptads)ectodomain (ECD); residues Arg215-Asn671	0.003	0.002	1.6	25 °C	Open record: ADAM17 (TACE), substrate 9 (α-helical, three α-helix-inducing heptads)
ADAM17 (TACE)	—	substrate 9 (α-helical, three α-helix-inducing heptads)catalytic domain (CD); residues Arg215-Val477	0.004	0.0023	1.6	25 °C	Open record: ADAM17 (TACE), substrate 9 (α-helical, three α-helix-inducing heptads)