Values CatLog read from this paper

Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA

PMID 23801116doi:10.1007/s10529-013-1262-yOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (60 values) from this paper: NfsA from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.6.5.10

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
NfsA	Escherichia coli	2,4,6-trinitrotoluene	56.21	0.029	1.94 × 10³	pH 7	Open record: NfsA, 2,4,6-trinitrotoluene
NfsA	Escherichia coli	2,4-dinitrotoluene	24.94	0.176	142	pH 7	Open record: NfsA, 2,4-dinitrotoluene
NfsA	Escherichia coli	5-(aziridin-1-yl)-2,4-dinitrobenzamide	36.64	0.327	112	pH 7	Open record: NfsA, 5-(aziridin-1-yl)-2,4-dinitrobenzamide
NfsA	Escherichia coli	NADPH	16.3	0.085	192	pH 7	Open record: NfsA, NADPH
NfsA	Escherichia coli	nitrofurazone	19.37	0.012	1.61 × 10³	pH 7	Open record: NfsA, nitrofurazone
NfsAvariant F42A	Escherichia coli	2,4,6-trinitrotoluene	0.67	0.013	50	pH 7	Open record: NfsA variant F42A, 2,4,6-trinitrotoluene
NfsAvariant F42A	Escherichia coli	2,4-dinitrotoluene	0.7	0.366	2	pH 7	Open record: NfsA variant F42A, 2,4-dinitrotoluene
NfsAvariant F42A	Escherichia coli	5-(aziridin-1-yl)-2,4-dinitrobenzamide	1.08	0.487	2	pH 7	Open record: NfsA variant F42A, 5-(aziridin-1-yl)-2,4-dinitrobenzamide
NfsAvariant F42A	Escherichia coli	NADPH	0.46	0.085	5	pH 7	Open record: NfsA variant F42A, NADPH
NfsAvariant F42A	Escherichia coli	nitrofurazone	0.8	0.064	10	pH 7	Open record: NfsA variant F42A, nitrofurazone
NfsAvariant F42N	Escherichia coli	2,4,6-trinitrotoluene	4.95	0.02	250	pH 7	Open record: NfsA variant F42N, 2,4,6-trinitrotoluene
NfsAvariant F42N	Escherichia coli	2,4-dinitrotoluene	3.64	0.313	12	pH 7	Open record: NfsA variant F42N, 2,4-dinitrotoluene
NfsAvariant F42N	Escherichia coli	5-(aziridin-1-yl)-2,4-dinitrobenzamide	2.99	0.332	9	pH 7	Open record: NfsA variant F42N, 5-(aziridin-1-yl)-2,4-dinitrobenzamide
NfsAvariant F42N	Escherichia coli	NADPH	0.78	0.049	16	pH 7	Open record: NfsA variant F42N, NADPH
NfsAvariant F42N	Escherichia coli	nitrofurazone	1.82	0.023	80	pH 7	Open record: NfsA variant F42N, nitrofurazone
NfsAvariant F42Y	Escherichia coli	2,4,6-trinitrotoluene	27.06	0.02	1.35 × 10³	pH 7	Open record: NfsA variant F42Y, 2,4,6-trinitrotoluene
NfsAvariant F42Y	Escherichia coli	2,4-dinitrotoluene	17.31	0.206	84	pH 7	Open record: NfsA variant F42Y, 2,4-dinitrotoluene
NfsAvariant F42Y	Escherichia coli	5-(aziridin-1-yl)-2,4-dinitrobenzamide	24.27	0.322	75	pH 7	Open record: NfsA variant F42Y, 5-(aziridin-1-yl)-2,4-dinitrobenzamide
NfsAvariant F42Y	Escherichia coli	NADPH	9.01	0.054	167	pH 7	Open record: NfsA variant F42Y, NADPH
NfsAvariant F42Y	Escherichia coli	nitrofurazone	15.73	0.022	720	pH 7	Open record: NfsA variant F42Y, nitrofurazone