Values CatLog read from this paper
DXP synthase-catalyzed C-N bond formation: nitroso substrate specificity studies guide selective inhibitor design
PMID 23824585doi:10.1002/cbic.201300187Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (38 values) from this paper: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), pyruvate dehydrogenase (PDH) E1 subunit from Escherichia coli, Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 1-methyl-2-nitrosobenzene | 0.6 | 0.099 | 6 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 1-methyl-2-nitrosobenzene |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 1-nitrosonaphthalen-2-ol | 0.018 | 0.041 | 0.45 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 2-hydroxy-4,6-dinitrobenzaldehyde | 0.0058 | 0.512 | 0.011 calc. | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 2-hydroxy-4,6-dinitrobenzaldehyde | |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole | 0.023 | 0.387 | 0.06 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 3,5-dimethyl-4-nitroso-1-(propan-2-yl)-1H-pyrazole |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 6-bromo-1-nitrosonaphthalen-2-ol | 0.033 | 0.024 | 1.3 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 6-bromo-1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | 0.0197 | 0.018 | 1.1 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | 7-methoxy-1-nitrosonaphthalen-2-ol | 0.022 | 0.063 | 0.35 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), 7-methoxy-1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | D-glyceraldehyde 3-phosphate | 1.7 | 0.028 | 60.7 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), D-glyceraldehyde 3-phosphate |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | N,N-dimethyl-4-nitrosoaniline | 0.015 | 0.054 | 0.28 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), N,N-dimethyl-4-nitrosoaniline |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) | Escherichia coli | nitrosobenzene | 2.92 | 0.133 | 22 | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS), nitrosobenzene |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R420A | Escherichia coli | 1-nitrosonaphthalen-2-ol | — | 0.032 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R420A, 1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R420A | Escherichia coli | 6-bromo-1-nitrosonaphthalen-2-ol | — | 0.016 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R420A, 6-bromo-1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R420A | Escherichia coli | 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | — | 0.014 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R420A, 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R478A | Escherichia coli | 1-nitrosonaphthalen-2-ol | — | 0.023 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R478A, 1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R478A | Escherichia coli | 6-bromo-1-nitrosonaphthalen-2-ol | — | 0.02 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R478A, 6-bromo-1-nitrosonaphthalen-2-ol |
| DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS)variant R478A | Escherichia coli | 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide | — | 0.013 | — | pH 8, 37 °C | Open record: DXP synthase (1-deoxy-D-xylulose 5-phosphate synthase, DXPS) variant R478A, 6-hydroxy-5-nitrosonaphthalene-2-sulfonamide |
| pyruvate dehydrogenase (PDH) E1 subunit | Sus scrofa | 6-bromo-1-nitrosonaphthalen-2-ol | — | 0.45 | — | pH 8, 37 °C | Open record: pyruvate dehydrogenase (PDH) E1 subunit, 6-bromo-1-nitrosonaphthalen-2-ol |
| pyruvate dehydrogenase (PDH) E1 subunit | Sus scrofa | N,N-dimethyl-4-nitrosoaniline | — | 0.408 | — | pH 8, 37 °C | Open record: pyruvate dehydrogenase (PDH) E1 subunit, N,N-dimethyl-4-nitrosoaniline |
| pyruvate dehydrogenase (PDH) E1 subunit | Sus scrofa | nitrosobenzene | — | 0.35 | — | pH 8, 37 °C | Open record: pyruvate dehydrogenase (PDH) E1 subunit, nitrosobenzene |