Values CatLog read from this paper
Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression
PMID 23843461doi:10.1074/jbc.m113.484733Open in Browse
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CatLog read 4 records (12 values) from this paper: lacto-N-biosidase LnbX (BLLJ_1505) from Bifidobacterium longum subsp. longum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lacto-N-biosidase LnbX (BLLJ_1505) | Bifidobacterium longum subsp. longum | lacto-N-fucopentaose I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc) | 13.5 | 14.6 | 0.923 | pH 5.4, 25 °C | Open record: lacto-N-biosidase LnbX (BLLJ_1505), lacto-N-fucopentaose I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc) |
| lacto-N-biosidase LnbX (BLLJ_1505) | Bifidobacterium longum subsp. longum | lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) | 113 | 0.401 | 282 | pH 5.4, 25 °C | Open record: lacto-N-biosidase LnbX (BLLJ_1505), lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) |
| lacto-N-biosidase LnbX (BLLJ_1505) | Bifidobacterium longum subsp. longum | p-nitrophenyl beta-lacto-N-bioside I (Galβ1-3GlcNAcβ-pNP) | 96.1 | 0.119 | 806 | pH 5.4, 25 °C | Open record: lacto-N-biosidase LnbX (BLLJ_1505), p-nitrophenyl beta-lacto-N-bioside I (Galβ1-3GlcNAcβ-pNP) |
| lacto-N-biosidase LnbX (BLLJ_1505) | Bifidobacterium longum subsp. longum | p-nitrophenyl GalNAcβ1-3GlcNAcβ (GalNAcβ1-3GlcNAcβ-pNP) | 39.4 | 0.186 | 211 | pH 5.4, 25 °C | Open record: lacto-N-biosidase LnbX (BLLJ_1505), p-nitrophenyl GalNAcβ1-3GlcNAcβ (GalNAcβ1-3GlcNAcβ-pNP) |