EC 3.2.1.140: lacto-N-biosidase

HydrolasesOpen in Browse

CatLog read 46 records for EC 3.2.1.140 from 5 papers, for 14 organisms. Names in these papers: lacto-N-biosidase (LnbB), lacto-N-biosidase LnbX (BLLJ_1505), lacto-N-biosidase LnbB, lacto-N-biosidase LnbX and 13 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 8 records for EC 3.2.1.140 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.140 in Browse.

Papers

Papers with records for EC 3.2.1.140
Paper	Organisms	Records
Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis	Bifidobacterium bifidum	14
Discovery of Lacto-N-Biosidases and a Novel N-Acetyllactosaminidase Activity in the CAZy Family GH20: Functional Diversity and Structural Insights	Actinomyces israelii, Aquimarina sp. and 7 more	14
Crystal structures of glycoside hydrolase family 136 lacto-N-biosidases from monkey gut- and human adult gut bacteria	Bifidobacterium longum subsp. longum, Bifidobacterium saguini and 2 more	8
Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1	Bifidobacterium bifidum, Bifidobacterium longum subsp. longum	6
Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression	Bifidobacterium longum subsp. longum	4

Records

Records for EC 3.2.1.140 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
AcisGH20_tr (GH20 lacto-N-biosidase)	Actinomyces israelii	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	95.1	0.098	1.03 × 10³	pH 5.2, 51 °C	PMID 39239753	Open record: AcisGH20_tr (GH20 lacto-N-biosidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Aquimarina sp.	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	2.16	0.507	4.25	pH 6, 35 °C	PMID 39239753	Open record: AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Aquimarina sp.	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	3.9	0.909	4.34	pH 6, 35 °C	PMID 39239753	Open record: AquiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
AralGH20_tr (GH20 lacto-N-biosidase)	Arthrobacter alpinus	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	5.14	0.1477	34.8	pH 7, 40 °C	PMID 39239753	Open record: AralGH20_tr (GH20 lacto-N-biosidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
BlLnbX lacto-N-biosidase (GH136)	Bifidobacterium longum subsp. longum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)	3.1	0.05	62	pH 7, 25 °C	PMID 35092420	Open record: BlLnbX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)
BlLnbX lacto-N-biosidase (GH136)	Bifidobacterium longum subsp. longum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)	52	0.164	318	pH 7, 25 °C	PMID 35092420	Open record: BlLnbX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)
BsaX lacto-N-biosidase (GH136)	Bifidobacterium saguini	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)	5.1	0.037	137	pH 7, 25 °C	PMID 35092420	Open record: BsaX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)
BsaX lacto-N-biosidase (GH136)	Bifidobacterium saguini	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)	45	0.114	399	pH 7, 25 °C	PMID 35092420	Open record: BsaX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)
lacto-N-biosidase (LnbB)	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	80	0.52	153	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB), p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	90	0.75	120	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB), p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant H263A	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	0.67	0.67	0.982	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant H263A, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant H263A	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	1.08	1.28	0.893	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant H263A, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant H263R	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	1	0.78	1.29	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant H263R, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant H263R	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	1	0.72	1.44	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant H263R, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant N259Q	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	0.034	0.235	0.145	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant N259Q, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant N259Q	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	0.07	0.49	0.15	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant N259Q, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant Q190L	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	0.201	0.034	5.93	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant Q190L, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant Q190L	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	0.41	0.078	5.24	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant Q190L, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant W394A	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	0.041	0.087	0.458	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant W394A, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant W394A	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	0.06	0.53	0.119	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant W394A, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant W394F	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor	0.186	0.26	0.718	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant W394F, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase (LnbB)variant W394F	Bifidobacterium bifidum	p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor	0.26	0.24	1.09	pH 4.5, 30 °C	PMID 33810098	Open record: lacto-N-biosidase (LnbB) variant W394F, p-nitrophenyl beta-lacto-N-bioside
lacto-N-biosidase LnbB	Bifidobacterium bifidum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside	4.9	—	—	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbB, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside
lacto-N-biosidase LnbB	Bifidobacterium bifidum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside	16	—	—	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbB, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside
lacto-N-biosidase LnbB	Bifidobacterium bifidum	Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc	0.029	3.3	0.0088	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbB, Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc
lacto-N-biosidase LnbX	Bifidobacterium longum subsp. longum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside	1.5	—	—	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbX, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside
lacto-N-biosidase LnbX	Bifidobacterium longum subsp. longum	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside	88	0.12	730	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbX, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside
lacto-N-biosidase LnbX	Bifidobacterium longum subsp. longum	Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc	0.84	2.5	0.34	pH 6, 30 °C	PMID 25839135	Open record: lacto-N-biosidase LnbX, Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc
lacto-N-biosidase LnbX (BLLJ_1505)	Bifidobacterium longum subsp. longum	lacto-N-fucopentaose I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc)	13.5	14.6	0.923	pH 5.4, 25 °C	PMID 23843461	Open record: lacto-N-biosidase LnbX (BLLJ_1505), lacto-N-fucopentaose I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc)
lacto-N-biosidase LnbX (BLLJ_1505)	Bifidobacterium longum subsp. longum	lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc)	113	0.401	282	pH 5.4, 25 °C	PMID 23843461	Open record: lacto-N-biosidase LnbX (BLLJ_1505), lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc)
lacto-N-biosidase LnbX (BLLJ_1505)	Bifidobacterium longum subsp. longum	p-nitrophenyl beta-lacto-N-bioside I (Galβ1-3GlcNAcβ-pNP)	96.1	0.119	806	pH 5.4, 25 °C	PMID 23843461	Open record: lacto-N-biosidase LnbX (BLLJ_1505), p-nitrophenyl beta-lacto-N-bioside I (Galβ1-3GlcNAcβ-pNP)
lacto-N-biosidase LnbX (BLLJ_1505)	Bifidobacterium longum subsp. longum	p-nitrophenyl GalNAcβ1-3GlcNAcβ (GalNAcβ1-3GlcNAcβ-pNP)	39.4	0.186	211	pH 5.4, 25 °C	PMID 23843461	Open record: lacto-N-biosidase LnbX (BLLJ_1505), p-nitrophenyl GalNAcβ1-3GlcNAcβ (GalNAcβ1-3GlcNAcβ-pNP)
MisoGH20_tr (GH20 lacto-N-biosidase)	Microlunatus soli	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	2.37	7.418	0.319	pH 4, 40 °C	PMID 39239753	Open record: MisoGH20_tr (GH20 lacto-N-biosidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Parabacteroides distasonis	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	14.6	6.866	2.12	pH 6, 50 °C	PMID 39239753	Open record: PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Parabacteroides distasonis	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	23.6	1.769	13.4	pH 6, 50 °C	PMID 39239753	Open record: PadiGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Paludibaculum fermentans	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	—	—	0.666	pH 6, 45 °C	PMID 39239753	Open record: PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Paludibaculum fermentans	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	17.1	1.408	13	pH 6, 45 °C	PMID 39239753	Open record: PafeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Planctomycetales bacterium	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	4.6	1.087	4.25	pH 6, 50 °C	PMID 39239753	Open record: PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Planctomycetales bacterium	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	8.9	1.775	4.99	pH 6, 50 °C	PMID 39239753	Open record: PlbaGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
RlX lacto-N-biosidase (GH136)	Ruminococcus lactaris	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)	7.7	0.092	83	pH 7, 25 °C	PMID 35092420	Open record: RlX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)
RlX lacto-N-biosidase (GH136)	Ruminococcus lactaris	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)	17	0.058	289	pH 7, 25 °C	PMID 35092420	Open record: RlX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)
TnX lacto-N-biosidase (GH136)	Tyzzerella nexilis	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)	3.9	0.099	40	pH 7, 25 °C	PMID 35092420	Open record: TnX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside (p-nitrophenyl-beta-galacto-N-bioside, Galβ1-3GalNAcβ-pNP)
TnX lacto-N-biosidase (GH136)	Tyzzerella nexilis	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)	27	0.133	201	pH 7, 25 °C	PMID 35092420	Open record: TnX lacto-N-biosidase (GH136), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside I, Galβ1-3GlcNAcβ-pNP)
TrdeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Treponema denticola	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	0.45	4.543	0.1	pH 6, 40 °C	PMID 39239753	Open record: TrdeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)
TrdeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase)	Treponema denticola	4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)	3.3	2.084	1.57	pH 6, 40 °C	PMID 39239753	Open record: TrdeGH20 (GH20 lacto-N-biosidase/N-acetyllactosaminidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-N-acetyllactosaminide; LacNAc = Gal-beta1,4-GlcNAc)
TrpyGH20_tr (GH20 lacto-N-biosidase)	Trueperella pyogenes	4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)	18.8	0.0498	380		PMID 39239753	Open record: TrpyGH20_tr (GH20 lacto-N-biosidase), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside (p-nitrophenyl-beta-lacto-N-bioside; LNB = Gal-beta1,3-GlcNAc)