Values CatLog read from this paper
Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis
PMID 33810098doi:10.3390/ijms22063230Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (42 values) from this paper: lacto-N-biosidase (LnbB) from Bifidobacterium bifidum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lacto-N-biosidase (LnbB) | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 80 | 0.52 | 153 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB), p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB) | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 90 | 0.75 | 120 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB), p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant H263A | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 0.67 | 0.67 | 0.982 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant H263A, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant H263A | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 1.08 | 1.28 | 0.893 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant H263A, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant H263R | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 1 | 0.78 | 1.29 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant H263R, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant H263R | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 1 | 0.72 | 1.44 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant H263R, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant N259Q | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 0.034 | 0.235 | 0.145 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant N259Q, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant N259Q | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 0.07 | 0.49 | 0.15 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant N259Q, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant Q190L | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 0.201 | 0.034 | 5.93 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant Q190L, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant Q190L | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 0.41 | 0.078 | 5.24 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant Q190L, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant W394A | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 0.041 | 0.087 | 0.458 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant W394A, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant W394A | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 0.06 | 0.53 | 0.119 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant W394A, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant W394F | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidehydrolysis mode; no acceptor | 0.186 | 0.26 | 0.718 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant W394F, p-nitrophenyl beta-lacto-N-bioside |
| lacto-N-biosidase (LnbB)variant W394F | Bifidobacterium bifidum | p-nitrophenyl beta-lacto-N-biosidetransglycosylation mode; 200 mM lactose acceptor | 0.26 | 0.24 | 1.09 | pH 4.5, 30 °C | Open record: lacto-N-biosidase (LnbB) variant W394F, p-nitrophenyl beta-lacto-N-bioside |