Values CatLog read from this paper
Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1
PMID 25839135doi:10.1016/j.carres.2015.03.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: lacto-N-biosidase LnbB, lacto-N-biosidase LnbX from Bifidobacterium bifidum, Bifidobacterium longum subsp. longum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lacto-N-biosidase LnbB | Bifidobacterium bifidum | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside | 4.9 | — | — | pH 6, 30 °C | Open record: lacto-N-biosidase LnbB, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside |
| lacto-N-biosidase LnbB | Bifidobacterium bifidum | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 16 | — | — | pH 6, 30 °C | Open record: lacto-N-biosidase LnbB, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| lacto-N-biosidase LnbB | Bifidobacterium bifidum | Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc | 0.029 | 3.3 | 0.0088 | pH 6, 30 °C | Open record: lacto-N-biosidase LnbB, Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc |
| lacto-N-biosidase LnbX | Bifidobacterium longum subsp. longum | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside | 1.5 | — | — | pH 6, 30 °C | Open record: lacto-N-biosidase LnbX, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-galactopyranoside |
| lacto-N-biosidase LnbX | Bifidobacterium longum subsp. longum | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside | 88 | 0.12 | 730 | pH 6, 30 °C | Open record: lacto-N-biosidase LnbX, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside |
| lacto-N-biosidase LnbX | Bifidobacterium longum subsp. longum | Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc | 0.84 | 2.5 | 0.34 | pH 6, 30 °C | Open record: lacto-N-biosidase LnbX, Gb5 pentasaccharide, Galβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glc |