Values CatLog read from this paper
The roles of active site residues in the catalytic mechanism of methylaspartate ammonia-lyase
PMID 23905011doi:10.1016/j.fob.2013.07.002Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (18 values) from this paper: methylaspartate ammonia-lyase (MAL) from Clostridium tetanomorphum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylaspartate ammonia-lyase (MAL)variant C361A | Clostridium tetanomorphum | mesaconate | 27.5 | 2.9 | 9.5 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant C361A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant F170A | Clostridium tetanomorphum | mesaconate | 10 | 0.9 | 11 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant F170A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant L384A | Clostridium tetanomorphum | mesaconate | — | — | 0.27 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant L384A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant Q172A | Clostridium tetanomorphum | mesaconate | — | — | 0.34 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant Q172A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant Q73A | Clostridium tetanomorphum | mesaconate | 0.7 | 0.2 | 3.5 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant Q73A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant T360A | Clostridium tetanomorphum | mesaconate | 0.6 | 15 | 0.04 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant T360A, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant T360S | Clostridium tetanomorphum | mesaconate | 35 | 1.4 | 25 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant T360S, mesaconate |
| methylaspartate ammonia-lyase (MAL)variant Y356A | Clostridium tetanomorphum | mesaconate | — | — | 0.012 | pH 9, 30 °C | Open record: methylaspartate ammonia-lyase (MAL) variant Y356A, mesaconate |