EC 4.3.1.2: methylaspartate ammonia-lyase

LyasesOpen in Browse

CatLog read 14 records for EC 4.3.1.2 from 2 papers, for 2 organisms. Names in these papers: methylaspartate ammonia-lyase (MAL), methylaspartate lyase (EcMAL).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 33 records for EC 4.3.1.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.3.1.2 in Browse.

Papers

Papers with records for EC 4.3.1.2
Paper	Organisms	Records
The roles of active site residues in the catalytic mechanism of methylaspartate ammonia-lyase	Clostridium tetanomorphum	8
Structure-guided protein engineering of ammonia lyase for efficient synthesis of sterically bulky unnatural amino acids	Escherichia coli O157:H7	6

Records

Records for EC 4.3.1.2 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
methylaspartate ammonia-lyase (MAL)variant C361A	Clostridium tetanomorphum	mesaconate	27.5	2.9	9.5	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant C361A, mesaconate
methylaspartate ammonia-lyase (MAL)variant F170A	Clostridium tetanomorphum	mesaconate	10	0.9	11	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant F170A, mesaconate
methylaspartate ammonia-lyase (MAL)variant L384A	Clostridium tetanomorphum	mesaconate	—	—	0.27	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant L384A, mesaconate
methylaspartate ammonia-lyase (MAL)variant Q172A	Clostridium tetanomorphum	mesaconate	—	—	0.34	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant Q172A, mesaconate
methylaspartate ammonia-lyase (MAL)variant Q73A	Clostridium tetanomorphum	mesaconate	0.7	0.2	3.5	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant Q73A, mesaconate
methylaspartate ammonia-lyase (MAL)variant T360A	Clostridium tetanomorphum	mesaconate	0.6	15	0.04	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant T360A, mesaconate
methylaspartate ammonia-lyase (MAL)variant T360S	Clostridium tetanomorphum	mesaconate	35	1.4	25	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant T360S, mesaconate
methylaspartate ammonia-lyase (MAL)variant Y356A	Clostridium tetanomorphum	mesaconate	—	—	0.012	pH 9, 30 °C	PMID 23905011	Open record: methylaspartate ammonia-lyase (MAL) variant Y356A, mesaconate
methylaspartate lyase (EcMAL)variant E172R/Y240S/D307L/Q329F/Y356S/C361L	Escherichia coli O157:H7	caffeic acid	1.029	1.32	0.78	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant E172R/Y240S/D307L/Q329F/Y356S/C361L, caffeic acid
methylaspartate lyase (EcMAL)variant E172R/Y240S/D307L/Q329F/Y356S/C361L	Escherichia coli O157:H7	cinnamic acid	0.5247	1.34	0.392	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant E172R/Y240S/D307L/Q329F/Y356S/C361L, cinnamic acid
methylaspartate lyase (EcMAL)variant F170S/E172R/D307L	Escherichia coli O157:H7	caffeic acid	0.575	1.47	0.392	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant F170S/E172R/D307L, caffeic acid
methylaspartate lyase (EcMAL)variant F170S/E172R/D307L	Escherichia coli O157:H7	cinnamic acid	0.708	1.23	0.577	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant F170S/E172R/D307L, cinnamic acid
methylaspartate lyase (EcMAL)variant Q329F/K331R/Y356S/C361L	Escherichia coli O157:H7	caffeic acid	0.767	1.25	0.613	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant Q329F/K331R/Y356S/C361L, caffeic acid
methylaspartate lyase (EcMAL)variant Q329F/K331R/Y356S/C361L	Escherichia coli O157:H7	cinnamic acid	0.358	1.96	0.183	pH 8.5	PMID 38650190	Open record: methylaspartate lyase (EcMAL) variant Q329F/K331R/Y356S/C361L, cinnamic acid