CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structure-guided protein engineering of ammonia lyase for efficient synthesis of sterically bulky unnatural amino acids

PMID 38650190doi:10.1186/s40643-021-00456-5Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 6 records (18 values) from this paper: methylaspartate lyase (EcMAL) from Escherichia coli O157:H7.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.3.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
methylaspartate lyase (EcMAL)variant E172R/Y240S/D307L/Q329F/Y356S/C361LEscherichia coli O157:H7caffeic acid1.0291.320.78pH 8.5Open
methylaspartate lyase (EcMAL)variant E172R/Y240S/D307L/Q329F/Y356S/C361LEscherichia coli O157:H7cinnamic acid0.52471.340.392pH 8.5Open
methylaspartate lyase (EcMAL)variant F170S/E172R/D307LEscherichia coli O157:H7caffeic acid0.5751.470.392pH 8.5Open
methylaspartate lyase (EcMAL)variant F170S/E172R/D307LEscherichia coli O157:H7cinnamic acid0.7081.230.577pH 8.5Open
methylaspartate lyase (EcMAL)variant Q329F/K331R/Y356S/C361LEscherichia coli O157:H7caffeic acid0.7671.250.613pH 8.5Open
methylaspartate lyase (EcMAL)variant Q329F/K331R/Y356S/C361LEscherichia coli O157:H7cinnamic acid0.3581.960.183pH 8.5Open