Values CatLog read from this paper
Enzymatic basis for N-glycan sialylation: structure of rat α2,6-sialyltransferase (ST6GAL1) reveals conserved and unique features for glycan sialylation
PMID 24155237doi:10.1074/jbc.m113.519041Open in Browse
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CatLog read 18 records (54 values) from this paper: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) from Rattus norvegicus (rat).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acidexpressed in HEK293-F cells; Ni-NTA purified | 0.593 | 0.092 | 6.45 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1), CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acidtags removed by TEV protease and N-glycans trimmed by EndoF1 | 0.49 | 0.093 | 5.27 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1), CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) | Rattus norvegicus (rat) | N-acetyllactosamineexpressed in HEK293-F cells; Ni-NTA purified | 0.672 | 1.8 | 0.373 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1), N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) | Rattus norvegicus (rat) | N-acetyllactosaminetags removed by TEV protease and N-glycans trimmed by EndoF1 | 0.35 | 1.4 | 0.25 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1), N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant D271A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.048 | 0.365 | 0.13 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant D271A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant D271A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.092 | 6.5 | 0.013 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant D271A, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant F208A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.188 | 0.4 | 0.47 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant F208A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant F208A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.43 | 1 | 0.438 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant F208A, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant K355A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.693 | 0.095 | 7.3 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant K355A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant K355A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.393 | 1.1 | 0.358 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant K355A, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Q232A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.103 | 0.133 | 0.78 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Q232A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Q232A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.135 | 2.6 | 0.052 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Q232A, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Q354A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.417 | 0.1 | 4.2 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Q354A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Q354A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.643 | 1.8 | 0.357 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Q354A, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Y119F | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.39 | 0.078 | 5 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Y119F, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Y119F | Rattus norvegicus (rat) | N-acetyllactosamine | 0.313 | 1.8 | 0.173 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Y119F, N-acetyllactosamine |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Y272A | Rattus norvegicus (rat) | CMP-N-acetylneuraminic acid | 0.05 | 0.22 | 0.23 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Y272A, CMP-N-acetylneuraminic acid |
| ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1)variant Y272A | Rattus norvegicus (rat) | N-acetyllactosamine | 0.073 | 3.5 | 0.022 | pH 6.5, 37 °C | Open record: ST6GAL1 (beta-galactoside alpha2,6-sialyltransferase 1) variant Y272A, N-acetyllactosamine |