Values CatLog read from this paper
A role for glutamate-333 of Saccharomyces cerevisiae cystathionine γ-lyase as a determinant of specificity
PMID 24291053doi:10.1016/j.bbapap.2013.11.012Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 33 records (87 values) from this paper: cystathionine γ-lyase (yCGL) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cystathionine50 mM potassium phosphate; 0.01–5.5 mM L-Cth | 1.51 | 0.71 | 2.1 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cystathionineMBP buffer (50 mM MOPS, 50 mM bicine, 50 mM proline); pH 7.2 | — | — | 2.18 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cystathionine | — | — | 2.7 | pH 7.6, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cysteine | 0.15 | 0.09 | 1.7 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cysteine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-homocysteine | — | — | 2.7 × 10⁻⁵ | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-homocysteine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0097 | 2.5 | 0.0038 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL), O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E333A | Saccharomyces cerevisiae | L-cystathionine | 0.78 | 12 | 0.065 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333A, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E333A | Saccharomyces cerevisiae | L-cysteine | 1.3 | 1.2 | 0.81 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333A, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E333A | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0058 | 2.2 | 0.0026 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333A, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E333D | Saccharomyces cerevisiae | L-cystathionine | 0.98 | 12 | 0.082 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333D, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E333D | Saccharomyces cerevisiae | L-cysteine | 0.24 | 1.8 | 0.13 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333D, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E333D | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0045 | 0.9 | 0.005 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333D, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E333Q | Saccharomyces cerevisiae | L-cystathionine | 0.48 | 13 | 0.036 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333Q, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E333Q | Saccharomyces cerevisiae | L-cysteine | 0.5 | 0.7 | 0.49 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333Q, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E333Q | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0102 | 5.3 | 0.0019 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333Q, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48A | Saccharomyces cerevisiae | L-cystathionine | 0.54 | 0.76 | 0.71 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48A | Saccharomyces cerevisiae | L-cysteine | 0.09 | 0.11 | 0.8 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48A | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0142 | 1.5 | 0.01 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48A/E333A | Saccharomyces cerevisiae | L-cystathionine | — | — | 0.0037 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A/E333A, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48A/E333A | Saccharomyces cerevisiae | L-cysteine | 0.11 | 0.5 | 0.21 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A/E333A, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48A/E333A | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0047 | 2.9 | 0.0016 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A/E333A, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48D | Saccharomyces cerevisiae | L-cystathionine | 1.4 | 0.87 | 1.59 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48D | Saccharomyces cerevisiae | L-cysteine | 0.32 | 0.4 | 0.72 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48D | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0053 | 1.4 | 0.0038 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48D/E333D | Saccharomyces cerevisiae | L-cystathionine | — | — | 0.0088 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D/E333D, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48D/E333D | Saccharomyces cerevisiae | L-cysteine | 0.029 | 0.5 | 0.06 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D/E333D, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48D/E333D | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0035 | 0.7 | 0.005 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D/E333D, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48Q | Saccharomyces cerevisiae | L-cystathionine | 1.29 | 0.9 | 1.4 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48Q | Saccharomyces cerevisiae | L-cysteine | 0.18 | 0.18 | 1.02 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48Q | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0124 | 4.4 | 0.0028 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q, O-acetyl-L-serine |
| cystathionine γ-lyase (yCGL)variant E48Q/E333Q | Saccharomyces cerevisiae | L-cystathionine | — | — | 0.0169 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q/E333Q, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48Q/E333Q | Saccharomyces cerevisiae | L-cysteine | 0.16 | 0.4 | 0.31 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q/E333Q, L-cysteine |
| cystathionine γ-lyase (yCGL)variant E48Q/E333Q | Saccharomyces cerevisiae | O-acetyl-L-serine | 0.0092 | 1.5 | 0.006 | pH 7.2, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48Q/E333Q, O-acetyl-L-serine |