CatLogenzyme kinetics catalog

Values CatLog read from this paper

Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cyclic adenosine and guanosine 3',5'-monophosphate

PMID 25050706doi:10.1021/bi500406hOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 36 records (36 values) from this paper: phosphodiesterase-1 (yPDE1) from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.4.17

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer—0.0988—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer21——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer——213pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer—0.1157—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer17.4——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer——150pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer—0.1175—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer11.8——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer——100pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer—0.1101—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer16.9——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer——153pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)—0.0812—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)14.3——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)——176pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)—0.0773—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)11.1——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeadenosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)——144pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer—0.0968—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer15.1——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate10 mM MgCl2 in assay buffer——156pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer—0.1048—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer10.8——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate0.01 mM ZnCl2 in assay buffer——103pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer—0.1913—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer14.3——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphate2 mM MnCl2 in assay buffer——75pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer—0.1047—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer11.8——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateno divalent metal ion added to assay buffer——113pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)—0.1474—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)12.1——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)——82pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)—0.1539—pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)11.7——pH 7.5Open
phosphodiesterase-1 (yPDE1)Saccharomyces cerevisiaeguanosine 3',5'-cyclic monophosphateprotein dialyzed three times against 50 mM Tris-HCl (pH 7.5)——76pH 7.5Open