Values CatLog read from this paper
Biochemical and kinetic characterization of the recombinant ADP-forming acetyl coenzyme A synthetase from the amitochondriate protozoan Entamoeba histolytica
PMID 25303954doi:10.1128/ec.00192-14Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (48 values) from this paper: ADP-forming acetyl-CoA synthetase (ACD) from Entamoeba histolytica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | acetate | 240 | 14 | 16 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), acetate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | acetyl coenzyme ADTNB (Ellman) assay of CoASH release; 50 mM Tris-HCl; pH 7.3 | 110 | 0.04 | 2.6 × 10³ | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | acetyl coenzyme A100 mM Tris-HCl pH 7.3; 5.5 mM glucose; 1 mM NADP+ | 130 | 0.017 | 7.6 × 10³ calc. | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | adenosine 5'-diphosphateDTNB (Ellman) assay of CoASH release; 50 mM Tris-HCl; pH 7.3 | 140 | 1.6 | 89 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | adenosine 5'-diphosphateDTNB (Ellman) assay of CoASH release; 50 mM Tris-HCl; pH 7.3 | 24 | 0.71 | 34 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | adenosine 5'-diphosphate100 mM Tris-HCl pH 7.3; 5.5 mM glucose; 1 mM NADP+ | 140 | 0.94 | 150 calc. | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | adenosine 5'-triphosphateacetyl-CoA-forming direction; fixed 100 mM acetate; 1 mM CoA | 320 | 12 | 27 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), adenosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | adenosine 5'-triphosphatepropionyl-CoA-forming direction; fixed 250 mM propionate | 240 | 7.2 | 33 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), adenosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | coenzyme Aacetyl-CoA-forming direction; fixed 100 mM acetate | 220 | 0.2 | 1.1 × 10³ | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), coenzyme A |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | coenzyme Apropionyl-CoA-forming direction; fixed 250 mM propionate | 260 | 1.6 | 160 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), coenzyme A |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | guanosine 5'-diphosphate | 170 | 1.9 | 90 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), guanosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | guanosine 5'-triphosphate | 180 | 10 | 18 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), guanosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | phosphateDTNB (Ellman) assay of CoASH release; 50 mM Tris-HCl; pH 7.3 | 120 | 1.8 | 65 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), phosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | phosphateDTNB (Ellman) assay of CoASH release; 50 mM Tris-HCl; pH 7.3 | 18 | 1.5 | 12 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), phosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | phosphate100 mM Tris-HCl pH 7.3; 5.5 mM glucose; 1 mM NADP+ | 180 | 2.1 | 86 calc. | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), phosphate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | propionate | 190 | 29 | 6.3 | pH 7.5, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), propionate |
| ADP-forming acetyl-CoA synthetase (ACD) | Entamoeba histolytica | propionyl coenzyme A | 21 | 0.032 | 650 | pH 7.3, 37 °C | Open record: ADP-forming acetyl-CoA synthetase (ACD), propionyl coenzyme A |