Values CatLog read from this paper
Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria
PMID 25401076doi:10.1186/s13568-014-0076-1Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 48 records (113 values) from this paper: D-lactate dehydrogenase (FNLDH, FN0511 protein), D-lactate dehydrogenase (PALDH, PA0927 protein), D-lactate dehydrogenase (ECLDH, ECD_01352 protein) from Fusobacterium nucleatum subsp. nucleatum, Pseudomonas aeruginosa, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | 2-oxobutanoate | 31 | — | — | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), 2-oxobutanoate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Glyoxylate | 100 | — | — | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Glyoxylate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Hydroxypyruvate | 50 | 12 | 4.2 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Hydroxypyruvate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | NADH | 640 | 0.055 | 1.2 × 10⁴ | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), NADH |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | NADHno effector | 710 | 0.088 | 8.1 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), NADH |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | NADHwith 5 mM FBP | 730 | 0.092 | 8 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), NADH |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | NADHwith 2.4 mM MgCl2 | 690 | 0.09 | 7.7 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), NADH |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Oxaloacetate | 1.1 × 10³ | 54 | 21 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Oxaloacetate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Pyruvate | 410 | — | — | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Pyruvate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Pyruvateno effector | 380 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Pyruvate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Pyruvatewith 5 mM FBP | 400 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Pyruvate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | Pyruvatewith 2.4 mM MgCl2 | 370 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), Pyruvate |
| D-lactate dehydrogenase (ECLDH, ECD_01352 protein) | Escherichia coli | pyruvate | 670 | 5.6 | 120 calc. | pH 7, 30 °C | Open record: D-lactate dehydrogenase (ECLDH, ECD_01352 protein), pyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | 2-oxobutanoate | 140 | 0.31 | 440 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), 2-oxobutanoate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | 2-oxohexanoate | 230 | 2.6 | 87 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), 2-oxohexanoate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | 2-oxopentanoate | 140 | 0.68 | 200 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), 2-oxopentanoate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | 3-methyl-2-oxobutanoate | 120 | 3.5 | 35 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), 3-methyl-2-oxobutanoate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | 4-methyl-2-oxopentanoate | 200 | 3.8 | 53 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), 4-methyl-2-oxopentanoate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Glyoxylate | 11 | 18 | 0.7 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Glyoxylate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Hydroxypyruvate | 160 | 18 | 8.7 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Hydroxypyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | NADH | 98 | 0.022 | 4.4 × 10³ | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), NADH |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | NADHno effector | 90 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), NADH |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | NADHwith 10 mM FBP | 80 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), NADH |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | NADHwith 5 mM MgCl2 | 110 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), NADH |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Oxaloacetate | 80 | 2.5 | 31 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Oxaloacetate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Phenylpyruvate | 120 | 11 | 11 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Phenylpyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Pyruvate | 80 | 0.34 | 230 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Pyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Pyruvateno effector | 87 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Pyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Pyruvatewith 10 mM FBP | 100 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Pyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | Pyruvatewith 5 mM MgCl2 | 80 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), Pyruvate |
| D-lactate dehydrogenase (FNLDH, FN0511 protein) | Fusobacterium nucleatum subsp. nucleatum | pyruvate | 79 | 0.39 | 200 calc. | pH 7, 30 °C | Open record: D-lactate dehydrogenase (FNLDH, FN0511 protein), pyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | 2-oxobutanoate | 100 | 0.64 | 150 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), 2-oxobutanoate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | 2-oxohexanoate | 160 | 5.8 | 27 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), 2-oxohexanoate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | 2-oxopentanoate | 69 | 4 | 18 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), 2-oxopentanoate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | 4-methyl-2-oxopentanoate | 39 | 12 | 3.2 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), 4-methyl-2-oxopentanoate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Glyoxylate | 880 | 6.8 | 130 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Glyoxylate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Hydroxypyruvate | 420 | 3 | 140 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Hydroxypyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | NADH | 310 | 0.019 | 1.7 × 10⁴ | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), NADH |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | NADHno effector | 270 | 0.035 | 7.7 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), NADH |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | NADHwith 10 mM FBP | 270 | 0.035 | 7.8 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), NADH |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | NADHwith 5 mM MgCl2 | 270 | 0.035 | 7.7 × 10³ | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), NADH |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Oxaloacetate | 410 | 1.7 | 230 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Oxaloacetate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Phenylpyruvate | 18 | 7.1 | 2.6 | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Phenylpyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Pyruvate | 400 | 0.1 | 4 × 10³ | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Pyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Pyruvateno effector | 240 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Pyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Pyruvatewith 10 mM FBP | 240 | — | — | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Pyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | Pyruvatewith 5 mM MgCl2 | 290 | 0.36 | 820 | pH 8, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), Pyruvate |
| D-lactate dehydrogenase (PALDH, PA0927 protein) | Pseudomonas aeruginosa | pyruvate | 410 | 0.074 | 5.5 × 10³ calc. | pH 7, 30 °C | Open record: D-lactate dehydrogenase (PALDH, PA0927 protein), pyruvate |