Values CatLog read from this paper
Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay
PMID 25408103doi:10.1039/c4ob02057jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (26 values) from this paper: Cps2L (glucose-1-phosphate thymidylyltransferase) from Streptococcus pneumoniae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | (alpha-D-glucopyranosyl)methylphosphonic acidcoupled assay: inosine 1 mM; PNP 1 µM; cosubstrate dTTP | 0.55 | 0.18 | 31 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), (alpha-D-glucopyranosyl)methylphosphonic acid |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | (alpha-D-glucopyranosyl)methylphosphonic acidcoupled assay: inosine 1 mM; PNP 1 µM; cosubstrate UTP | 0.01 | 0.38 | 0.28 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), (alpha-D-glucopyranosyl)methylphosphonic acid |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | (alpha-D-glucopyranosyl)methylphosphonic acidrates fit to a competitive substrate model (Fig. 2e) | — | — | 80 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), (alpha-D-glucopyranosyl)methylphosphonic acid |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | 2-amino-2-deoxy-alpha-D-glucopyranosyl phosphatedTTP; cosubstrate dTTP | 4.5 | 0.69 | 6.5 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), 2-amino-2-deoxy-alpha-D-glucopyranosyl phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | 2-amino-2-deoxy-alpha-D-glucopyranosyl phosphateUTP; cosubstrate UTP | 0.1 | 0.7 | 0.15 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), 2-amino-2-deoxy-alpha-D-glucopyranosyl phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | 3-amino-3-deoxy-alpha-D-glucopyranosyl phosphate | 0.08 | 1.1 | 0.072 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), 3-amino-3-deoxy-alpha-D-glucopyranosyl phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | 3-azido-3-deoxy-alpha-D-glucopyranosyl phosphate | 3 × 10⁻⁴ | 1.1 | 2.7 × 10⁻⁴ | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), 3-azido-3-deoxy-alpha-D-glucopyranosyl phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | alpha-D-glucose 1-phosphatecoupled assay: inosine 1 mM; PNP 1 µM; cosubstrate dTTP | 24 | 0.083 | 280 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), alpha-D-glucose 1-phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | alpha-D-glucose 1-phosphatecoupled assay: inosine 1 mM; PNP 1 µM; cosubstrate UTP | 0.016 | 0.16 | 9.6 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), alpha-D-glucose 1-phosphate |
| Cps2L (glucose-1-phosphate thymidylyltransferase) | Streptococcus pneumoniae | alpha-D-glucose 1-phosphaterates fit to a competitive substrate model (Fig. 2e) | — | — | 830 | pH 7.5 | Open record: Cps2L (glucose-1-phosphate thymidylyltransferase), alpha-D-glucose 1-phosphate |