CatLogenzyme kinetics catalog

Values CatLog read from this paper

Single arginine mutation in two yeast isocitrate dehydrogenases: biochemical characterization and functional implication

PMID 25502799doi:10.1371/journal.pone.0115025Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (52 values) from this paper: NADP+-IDH1 (ScIDH1), IDH1 (HcIDH), NADP+-IDH (YlIDH) from Saccharomyces cerevisiae, Homo sapiens, Yarrowia lipolytica.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.42

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
IDH1 (HcIDH)Homo sapiensD-isocitrate15.60.00572.7 × 10³pH 8, 25 °COpen
IDH1 (HcIDH)Homo sapiensNADP+—0.0114—pH 8, 25 °COpen
IDH1 (HcIDH)variant R132AHomo sapiensD-isocitrate0.221.5400.1pH 8, 25 °COpen
IDH1 (HcIDH)variant R132AHomo sapiensNADP+—0.0181—pH 8, 25 °COpen
IDH1 (HcIDH)variant R132HHomo sapiensalpha-ketoglutarate0.240.15672pH 8, 25 °COpen
IDH1 (HcIDH)variant R132HHomo sapiensD-isocitrate0.412.2820.2pH 8, 25 °COpen
IDH1 (HcIDH)variant R132HHomo sapiensNADP+—0.0158—pH 8, 25 °COpen
IDH1 (HcIDH)variant R132HHomo sapiensNADPH—0.0067—pH 8, 25 °COpen
NADP+-IDH (YlIDH)Yarrowia lipolyticaD-isocitrate26.60.01172.3 × 10³pH 8, 25 °COpen
NADP+-IDH (YlIDH)Yarrowia lipolyticaNADP+—0.0041—pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141AYarrowia lipolyticaD-isocitrate1.20.36723pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141AYarrowia lipolyticaNADP+—0.0096—pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141HYarrowia lipolyticaalpha-ketoglutarate0.430.04739pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141HYarrowia lipolyticaD-isocitrate0.140.02565pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141HYarrowia lipolyticaNADP+—0.0153—pH 8, 25 °COpen
NADP+-IDH (YlIDH)variant R141HYarrowia lipolyticaNADPH—0.0032—pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)Saccharomyces cerevisiaeD-isocitrate26.30.01052.5 × 10³pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)Saccharomyces cerevisiaeNADP+—0.0174—pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148ASaccharomyces cerevisiaeD-isocitrate12.42.2635pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148ASaccharomyces cerevisiaeNADP+—0.0257—pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148ESaccharomyces cerevisiaeD-isocitrate0.621.2960.5pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148ESaccharomyces cerevisiaeNADP+—0.0232—pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148HSaccharomyces cerevisiaealpha-ketoglutarate0.340.0311pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148HSaccharomyces cerevisiaeD-isocitrate0.270.024811pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148HSaccharomyces cerevisiaeNADP+—0.0356—pH 8, 25 °COpen
NADP+-IDH1 (ScIDH1)variant R148HSaccharomyces cerevisiaeNADPH—0.0059—pH 8, 25 °COpen