Values CatLog read from this paper
Exploring allosteric activation of LigAB from Sphingobium sp. strain SYK-6 through kinetics, mutagenesis and computational studies
PMID 25562402doi:10.1016/j.abb.2014.12.019Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (22 values) from this paper: LigAB (protocatechuate 4,5-dioxygenase) from Sphingobium sp. SYK-6.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| LigAB (protocatechuate 4,5-dioxygenase) | Sphingobium sp. SYK-6 | protocatechuateno effector | 178 | 0.081 | 2.2 × 10³ | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase), protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase) | Sphingobium sp. SYK-6 | protocatechuatewith 5 mM vanillin (allosteric activator) | 230 | 0.13 | 1.8 × 10³ | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase), protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase)variant A18W | Sphingobium sp. SYK-6 | protocatechuate | 122 | 0.066 | 1.9 × 10³ | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant A18W, protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase)variant F103H | Sphingobium sp. SYK-6 | protocatechuate | 110 | 0.108 | 1.1 × 10³ | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant F103H, protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase)variant F103L | Sphingobium sp. SYK-6 | protocatechuate | 33 | 1.6 | 21 | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant F103L, protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase)variant F103T | Sphingobium sp. SYK-6 | protocatechuate | 112 | 0.21 | 530 | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant F103T, protocatechuate |
| LigAB (protocatechuate 4,5-dioxygenase)variant F103V | Sphingobium sp. SYK-6 | dioxygen | — | 0.094 | — | pH 7.5 | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant F103V, dioxygen |
| LigAB (protocatechuate 4,5-dioxygenase)variant F103V | Sphingobium sp. SYK-6 | protocatechuate | 89 | 0.26 | 340 | pH 7.5, 25 °C | Open record: LigAB (protocatechuate 4,5-dioxygenase) variant F103V, protocatechuate |