Values CatLog read from this paper
Structural basis of the stereospecificity of bacterial B12-dependent 2-hydroxyisobutyryl-CoA mutase
PMID 25720495doi:10.1074/jbc.m115.645689Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (54 values) from this paper: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) from Aquincola tertiaricarbonis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) | Aquincola tertiaricarbonis | 3-methylbutanoyl-CoA | 0.0113 | 0.288 | 0.04 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB), 3-methylbutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) | Aquincola tertiaricarbonis | pivaloyl-CoA | 0.0163 | 0.374 | 0.043 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB), pivaloyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117A | Aquincola tertiaricarbonis | (R)-3-hydroxybutanoyl-CoA | 0.0273 | 0.0075 | 3.67 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117A, (R)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117A | Aquincola tertiaricarbonis | (S)-3-hydroxybutanoyl-CoA | 0.0255 | 0.0146 | 1.75 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117A, (S)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117A | Aquincola tertiaricarbonis | 2-hydroxyisobutanoyl-CoA | 0.0443 | 0.042 | 1.053 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117A, 2-hydroxyisobutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117V | Aquincola tertiaricarbonis | (R)-3-hydroxybutanoyl-CoA | 0.173 | 0.067 | 2.58 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117V, (R)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117V | Aquincola tertiaricarbonis | (S)-3-hydroxybutanoyl-CoA | 0.0615 | 0.117 | 0.525 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117V, (S)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117V | Aquincola tertiaricarbonis | 2-hydroxyisobutanoyl-CoA | 0.0447 | 0.0614 | 0.727 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117V, 2-hydroxyisobutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant D117V | Aquincola tertiaricarbonis | pivaloyl-CoA | 0.1395 | 0.0435 | 3.2 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant D117V, pivaloyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90A | Aquincola tertiaricarbonis | (R)-3-hydroxybutanoyl-CoA | 0.0035 | 0.298 | 0.012 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90A, (R)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90A | Aquincola tertiaricarbonis | (S)-3-hydroxybutanoyl-CoA | 0.002 | 0.433 | 0.0047 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90A, (S)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90A | Aquincola tertiaricarbonis | 2-hydroxyisobutanoyl-CoA | 0.0245 | 0.304 | 0.082 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90A, 2-hydroxyisobutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90A | Aquincola tertiaricarbonis | 3-methylbutanoyl-CoA | 0.009 | 0.147 | 0.062 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90A, 3-methylbutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90A | Aquincola tertiaricarbonis | pivaloyl-CoA | 0.0132 | 0.235 | 0.057 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90A, pivaloyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90L | Aquincola tertiaricarbonis | (S)-3-hydroxybutanoyl-CoA | 0.0035 | 0.663 | 0.0052 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90L, (S)-3-hydroxybutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90L | Aquincola tertiaricarbonis | 2-hydroxyisobutanoyl-CoA | 0.0067 | 0.517 | 0.013 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90L, 2-hydroxyisobutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90V | Aquincola tertiaricarbonis | 3-methylbutanoyl-CoA | 0.0093 | 0.329 | 0.028 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90V, 3-methylbutanoyl-CoA |
| 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB)variant I90V | Aquincola tertiaricarbonis | pivaloyl-CoA | 0.017 | 0.977 | 0.017 | pH 6.6, 30 °C | Open record: 2-hydroxyisobutyryl-CoA mutase (HCM, HcmA/HcmB) variant I90V, pivaloyl-CoA |