CatLogenzyme kinetics catalog

Values CatLog read from this paper

Nucleotide triphosphate promiscuity in Mycobacterium tuberculosis dethiobiotin synthetase

PMID 25801336doi:10.1016/j.tube.2015.02.046Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (56 values) from this paper: dethiobiotin synthetase (EcDTBS), dethiobiotin synthetase (MtDTBS) from Escherichia coli, Mycobacterium tuberculosis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 28 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.3.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dethiobiotin synthetase (EcDTBS)Escherichia coli(7R,8S)-7,8-diaminopelargonic acid0.0490.01523.2 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)Escherichia coliadenosine 5'-triphosphate0.03860.01053.68 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)Escherichia colicytidine 5'-triphosphate0.02811.0900.0258 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)Escherichia coliguanosine 5'-triphosphate0.0291.5610.019 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)Escherichia colisodium bicarbonate0.03840.59980.0640 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coli(7R,8S)-7,8-diaminopelargonic acid9 × 10⁻⁴0.01880.048 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coliadenosine 5'-triphosphate0.01810.01231.47 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia colicytidine 5'-triphosphate0.01910.39090.0489 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coliguanosine 5'-triphosphate0.01831.0970.0167 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia colisodium bicarbonate0.01620.4330.0374 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coli(7R,8S)-7,8-diaminopelargonic acid0.02320.0181.3 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coliadenosine 5'-triphosphate0.02360.01731.36 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia colicytidine 5'-triphosphate0.01960.8690.0226 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coliguanosine 5'-triphosphate0.01631.3870.0118 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia colisodium bicarbonate0.01540.38360.0401 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosis(7R,8S)-7,8-diaminopelargonic acid0.01330.03020.440 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisadenosine 5'-triphosphate0.01170.03020.387 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosiscytidine 5'-triphosphate0.01520.02520.603 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisguanosine 5'-triphosphate0.01110.02630.422 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisinosine 5'-triphosphate0.01020.01770.576 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosissodium bicarbonate0.00993.0740.0032 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisTTP0.01180.02320.509 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisuridine 5'-triphosphate0.01140.02610.437 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosis(7R,8S)-7,8-diaminopelargonic acid9 × 10⁻⁴0.01510.060 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosisadenosine 5'-triphosphate0.00130.02150.060 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosiscytidine 5'-triphosphate0.0010.04510.022 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosisguanosine 5'-triphosphate0.00120.10070.012 calc.pH 7.5, 37 °COpen
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosissodium bicarbonate0.001710.381.6 × 10⁻⁴ calc.pH 7.5, 37 °COpen