CatLogenzyme kinetics catalog

EC 6.3.3.3: dethiobiotin synthase

LigasesOpen in Browse

CatLog read 55 records for EC 6.3.3.3 from 3 papers, for 2 organisms. Names in these papers: dethiobiotin synthetase, dethiobiotin synthetase (EcDTBS), dethiobiotin synthetase (MtDTBS), dethiobiotin synthetase (DTBS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 18 records for EC 6.3.3.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.3.3 in Browse.

Papers

PaperOrganismsRecords
Nucleotide triphosphate promiscuity in Mycobacterium tuberculosis dethiobiotin synthetaseEscherichia coli, Mycobacterium tuberculosis28
Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural propertiesEscherichia coli25
Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetaseMycobacterium tuberculosis2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
dethiobiotin synthetaseEscherichia coli(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP0.06223 × 10⁻⁴207pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetaseEscherichia coli(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO30.02780.003158.84 calc.pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetaseEscherichia coliadenosine 5'-triphosphate—3.9 × 10⁻⁴159pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12AEscherichia coli(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP0.07680.0011368.2pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12AEscherichia coli(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO30.04320.0012933.5 calc.pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12AEscherichia coliadenosine 5'-triphosphate—3.3 × 10⁻⁴233pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12DEscherichia coli(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP0.11622.3 × 10⁻⁴505pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12DEscherichia coli(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO30.02980.0028810.4 calc.pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant E12DEscherichia coliadenosine 5'-triphosphate—0.0023848.8pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K15QEscherichia coli(7R,8S)-7,8-diaminononanoic acid9 × 10⁻⁶0.01118.1 × 10⁻⁴pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K15QEscherichia coliadenosine 5'-triphosphate—0.7011.29 × 10⁻⁵pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37LEscherichia coli(7R,8S)-7,8-diaminononanoic acid4.3 × 10⁻⁴0.03080.0141pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37LEscherichia coliadenosine 5'-triphosphate—0.00430.101pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37QEscherichia coli(7R,8S)-7,8-diaminononanoic acid5.3 × 10⁻⁴0.03040.0175pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37QEscherichia coliadenosine 5'-triphosphate—0.004530.118pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37REscherichia coli(7R,8S)-7,8-diaminononanoic acid4.8 × 10⁻⁵0.1433.38 × 10⁻⁴pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant K37REscherichia coliadenosine 5'-triphosphate—0.007440.0065pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41AEscherichia coli(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP0.06382.8 × 10⁻⁴228pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41AEscherichia coli(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO30.02820.01162.43 calc.pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41AEscherichia coliadenosine 5'-triphosphate—0.0022428.5pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41CEscherichia coli(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP0.05480.0016832.7pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41CEscherichia coli(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO30.02980.01971.51 calc.pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant S41CEscherichia coliadenosine 5'-triphosphate—0.0014537.8pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant T11VEscherichia coli(7R,8S)-7,8-diaminononanoic acid0.02523.4 × 10⁻⁴74pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetasevariant T11VEscherichia coliadenosine 5'-triphosphate—9.470.00265pH 7.5, 25 °CPMID 9125495Open
dethiobiotin synthetase (DTBS)Mycobacterium tuberculosis7,8-diaminopelargonic acid—0.002—pH 7.5PMID 20565114Open
dethiobiotin synthetase (DTBS)Mycobacterium tuberculosisATP—0.029—pH 7.5PMID 20565114Open
dethiobiotin synthetase (EcDTBS)Escherichia coli(7R,8S)-7,8-diaminopelargonic acid0.0490.01523.2 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)Escherichia coliadenosine 5'-triphosphate0.03860.01053.68 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)Escherichia colicytidine 5'-triphosphate0.02811.0900.0258 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)Escherichia coliguanosine 5'-triphosphate0.0291.5610.019 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)Escherichia colisodium bicarbonate0.03840.59980.0640 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coli(7R,8S)-7,8-diaminopelargonic acid9 × 10⁻⁴0.01880.048 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coliadenosine 5'-triphosphate0.01810.01231.47 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia colicytidine 5'-triphosphate0.01910.39090.0489 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia coliguanosine 5'-triphosphate0.01831.0970.0167 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175AEscherichia colisodium bicarbonate0.01620.4330.0374 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coli(7R,8S)-7,8-diaminopelargonic acid0.02320.0181.3 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coliadenosine 5'-triphosphate0.02360.01731.36 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia colicytidine 5'-triphosphate0.01960.8690.0226 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia coliguanosine 5'-triphosphate0.01631.3870.0118 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (EcDTBS)variant N175GEscherichia colisodium bicarbonate0.01540.38360.0401 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosis(7R,8S)-7,8-diaminopelargonic acid0.01330.03020.440 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisadenosine 5'-triphosphate0.01170.03020.387 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosiscytidine 5'-triphosphate0.01520.02520.603 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisguanosine 5'-triphosphate0.01110.02630.422 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisinosine 5'-triphosphate0.01020.01770.576 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosissodium bicarbonate0.00993.0740.0032 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisTTP0.01180.02320.509 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)Mycobacterium tuberculosisuridine 5'-triphosphate0.01140.02610.437 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosis(7R,8S)-7,8-diaminopelargonic acid9 × 10⁻⁴0.01510.060 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosisadenosine 5'-triphosphate0.00130.02150.060 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosiscytidine 5'-triphosphate0.0010.04510.022 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosisguanosine 5'-triphosphate0.00120.10070.012 calc.pH 7.5, 37 °CPMID 25801336Open
dethiobiotin synthetase (MtDTBS)variant G169NMycobacterium tuberculosissodium bicarbonate0.001710.381.6 × 10⁻⁴ calc.pH 7.5, 37 °CPMID 25801336Open