Values CatLog read from this paper

Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties

PMID 9125495doi:10.1021/bi9631677Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 25 records (60 values) from this paper: dethiobiotin synthetase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.3.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dethiobiotin synthetase	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP	0.0622	3 × 10⁻⁴	207	pH 7.5, 25 °C	Open record: dethiobiotin synthetase, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetase	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO3	0.0278	0.00315	8.84 calc.	pH 7.5, 25 °C	Open record: dethiobiotin synthetase, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetase	Escherichia coli	adenosine 5'-triphosphate	—	3.9 × 10⁻⁴	159	pH 7.5, 25 °C	Open record: dethiobiotin synthetase, adenosine 5'-triphosphate
dethiobiotin synthetasevariant E12A	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP	0.0768	0.00113	68.2	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12A, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant E12A	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO3	0.0432	0.00129	33.5 calc.	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12A, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant E12A	Escherichia coli	adenosine 5'-triphosphate	—	3.3 × 10⁻⁴	233	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12A, adenosine 5'-triphosphate
dethiobiotin synthetasevariant E12D	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP	0.1162	2.3 × 10⁻⁴	505	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12D, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant E12D	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO3	0.0298	0.00288	10.4 calc.	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12D, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant E12D	Escherichia coli	adenosine 5'-triphosphate	—	0.00238	48.8	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant E12D, adenosine 5'-triphosphate
dethiobiotin synthetasevariant K15Q	Escherichia coli	(7R,8S)-7,8-diaminononanoic acid	9 × 10⁻⁶	0.0111	8.1 × 10⁻⁴	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K15Q, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant K15Q	Escherichia coli	adenosine 5'-triphosphate	—	0.701	1.29 × 10⁻⁵	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K15Q, adenosine 5'-triphosphate
dethiobiotin synthetasevariant K37L	Escherichia coli	(7R,8S)-7,8-diaminononanoic acid	4.3 × 10⁻⁴	0.0308	0.0141	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37L, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant K37L	Escherichia coli	adenosine 5'-triphosphate	—	0.0043	0.101	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37L, adenosine 5'-triphosphate
dethiobiotin synthetasevariant K37Q	Escherichia coli	(7R,8S)-7,8-diaminononanoic acid	5.3 × 10⁻⁴	0.0304	0.0175	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37Q, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant K37Q	Escherichia coli	adenosine 5'-triphosphate	—	0.00453	0.118	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37Q, adenosine 5'-triphosphate
dethiobiotin synthetasevariant K37R	Escherichia coli	(7R,8S)-7,8-diaminononanoic acid	4.8 × 10⁻⁵	0.143	3.38 × 10⁻⁴	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37R, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant K37R	Escherichia coli	adenosine 5'-triphosphate	—	0.00744	0.0065	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant K37R, adenosine 5'-triphosphate
dethiobiotin synthetasevariant S41A	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP	0.0638	2.8 × 10⁻⁴	228	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41A, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant S41A	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO3	0.0282	0.0116	2.43 calc.	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41A, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant S41A	Escherichia coli	adenosine 5'-triphosphate	—	0.00224	28.5	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41A, adenosine 5'-triphosphate
dethiobiotin synthetasevariant S41C	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsaturating fixed substrates (CO2 as 15 mM NaHCO3); ATP	0.0548	0.00168	32.7	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41C, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant S41C	Escherichia coli	(7R,8S)-7,8-diaminononanoic acidsubsaturating ATP (1 µM); CO2 as 15 mM NaHCO3	0.0298	0.0197	1.51 calc.	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41C, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant S41C	Escherichia coli	adenosine 5'-triphosphate	—	0.00145	37.8	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant S41C, adenosine 5'-triphosphate
dethiobiotin synthetasevariant T11V	Escherichia coli	(7R,8S)-7,8-diaminononanoic acid	0.0252	3.4 × 10⁻⁴	74	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant T11V, (7R,8S)-7,8-diaminononanoic acid
dethiobiotin synthetasevariant T11V	Escherichia coli	adenosine 5'-triphosphate	—	9.47	0.00265	pH 7.5, 25 °C	Open record: dethiobiotin synthetase variant T11V, adenosine 5'-triphosphate