Values CatLog read from this paper
An unprecedented NADPH domain conformation in lysine monooxygenase NbtG provides insights into uncoupling of oxygen consumption from substrate hydroxylation
PMID 25802330doi:10.1074/jbc.m114.629485Open in Browse
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CatLog read 20 records (56 values) from this paper: lysine monooxygenase NbtG from Nocardia farcinica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lysine monooxygenase NbtG | Nocardia farcinica | D-Lyscosubstrate NADPH 2 mM | — | 2.6 | 0.1 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, D-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | D-Lyscosubstrate NADH 2 mM | — | 4.2 | 0.07 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, D-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | L-Lyscosubstrate NADPH 2 mM | — | 0.35 | 0.91 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, L-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | L-Lyscosubstrate NADH 2 mM | — | 0.9 | 0.58 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, L-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); no Lys; Table 2 | 0.32 | 0.36 | 0.9 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); cosubstrate L-Lys 2 mM | 1.29 | 0.4 | 3.3 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); cosubstrate D-Lys 10 mM | 2.5 | 1.6 | 1.5 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.62 | 0.6 | 1.1 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.31 | 3.8 | 0.08 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); no Lys; Table 2 | 0.58 | 0.2 | 2.9 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); cosubstrate L-Lys 2 mM | 1.08 | 0.14 | 7.7 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); cosubstrate D-Lys 10 mM | 2.9 | 0.51 | 5.6 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.32 | 0.7 | 0.46 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.41 | 0.5 | 0.52 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | D-Lyscosubstrate NADPH 2 mM | 0.13 | 49 | 0.0027 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant K64A, D-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | D-Lyscosubstrate NADH 2 mM | 0.021 | 62 | 3.8 × 10⁻⁴ | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant K64A, D-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | L-Lyscosubstrate NADPH 2 mM | 0.34 | 13 | 0.027 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant K64A, L-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | L-Lyscosubstrate NADH 2 mM | 0.18 | 40 | 0.0044 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant K64A, L-Lys |
| lysine monooxygenase NbtGvariant P238R | Nocardia farcinica | NADPHO2 consumption assay | 0.6 | 0.8 | 0.79 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant P238R, NADPH |
| lysine monooxygenase NbtGvariant P238R | Nocardia farcinica | NADPHL-Lys hydroxylation assay | 0.146 | 0.7 | 0.21 | pH 7.5, 25 °C | Open record: lysine monooxygenase NbtG variant P238R, NADPH |