EC 1.14.13.59: L-lysine N6-monooxygenase (NADPH)
OxidoreductasesOpen in Browse
CatLog read 34 records for EC 1.14.13.59 from 2 papers, for 1 organism. Names in these papers: lysine monooxygenase NbtG, L-lysine N6-monooxygenase (NbtG).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 12 records for EC 1.14.13.59 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.13.59 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| An unprecedented NADPH domain conformation in lysine monooxygenase NbtG provides insights into uncoupling of oxygen consumption from substrate hydroxylation | Nocardia farcinica | 20 |
| Identification of structural determinants of NAD(P)H selectivity and lysine binding in lysine N(6)-monooxygenase | Nocardia farcinica | 14 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | L-lysine | — | 11 | 0.09 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, L-lysine |
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | NADHno L-Lys (oxidase activity) | 1.3 | 2 | 0.67 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, NADH |
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | NADH2 mM L-Lys; cosubstrate L-Lys (2 mM) | 2.4 | 2.6 | 0.94 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, NADH |
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | NADPHoxygen consumption assay (Oxygraph) | 1.7 | 0.38 | 4.5 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, NADPH |
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | NADPHoxygen consumption assay (Oxygraph); 2 mM L-Lys | 2.4 | 0.4 | 5.95 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, NADPH |
| L-lysine N6-monooxygenase (NbtG)variant E216Q | Nocardia farcinica | NADPHfit with substrate inhibition (K_I printed); NADPH varied | 0.62 | 0.9 | 0.7 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant E216Q, NADPH |
| L-lysine N6-monooxygenase (NbtG)variant M239R | Nocardia farcinica | NADH | 0.7 | 0.08 | 8.9 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant M239R, NADH |
| L-lysine N6-monooxygenase (NbtG)variant M239R | Nocardia farcinica | NADPHoxygen consumption assay (Oxygraph) | 0.98 | 0.09 | 10.7 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant M239R, NADPH |
| L-lysine N6-monooxygenase (NbtG)variant M239R | Nocardia farcinica | NADPHfit with substrate inhibition (K_I printed); NADPH varied | 0.013 | 0.08 | 0.16 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant M239R, NADPH |
| L-lysine N6-monooxygenase (NbtG)variant R301A | Nocardia farcinica | L-lysine | — | 0.5 | 0.54 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant R301A, L-lysine |
| L-lysine N6-monooxygenase (NbtG)variant R301A | Nocardia farcinica | NADHoxygen consumption assay (Oxygraph) | 0.54 | 2.1 | 0.255 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant R301A, NADH |
| L-lysine N6-monooxygenase (NbtG)variant R301A | Nocardia farcinica | NADHoxygen consumption assay (Oxygraph); 2 mM L-Lys | 0.8 | 1.5 | 0.545 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant R301A, NADH |
| L-lysine N6-monooxygenase (NbtG)variant R301A | Nocardia farcinica | NADHfit with substrate inhibition (K_I printed); NADH varied | 0.28 | 2.1 | 0.13 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant R301A, NADH |
| L-lysine N6-monooxygenase (NbtG)variant R301A | Nocardia farcinica | NADPH | — | — | 0.026 | pH 7.5, 25 °C | PMID 27503802 | Open record: L-lysine N6-monooxygenase (NbtG) variant R301A, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | D-Lyscosubstrate NADPH 2 mM | — | 2.6 | 0.1 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, D-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | D-Lyscosubstrate NADH 2 mM | — | 4.2 | 0.07 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, D-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | L-Lyscosubstrate NADPH 2 mM | — | 0.35 | 0.91 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, L-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | L-Lyscosubstrate NADH 2 mM | — | 0.9 | 0.58 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, L-Lys |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); no Lys; Table 2 | 0.32 | 0.36 | 0.9 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); cosubstrate L-Lys 2 mM | 1.29 | 0.4 | 3.3 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHO2 consumption assay (Oxygraph); cosubstrate D-Lys 10 mM | 2.5 | 1.6 | 1.5 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.62 | 0.6 | 1.1 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.31 | 3.8 | 0.08 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); no Lys; Table 2 | 0.58 | 0.2 | 2.9 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); cosubstrate L-Lys 2 mM | 1.08 | 0.14 | 7.7 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHO2 consumption assay (Oxygraph); cosubstrate D-Lys 10 mM | 2.9 | 0.51 | 5.6 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.32 | 0.7 | 0.46 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtG | Nocardia farcinica | NADPHLys hydroxylation (Csaky iodine oxidation assay); Table 3 | 0.41 | 0.5 | 0.52 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG, NADPH |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | D-Lyscosubstrate NADPH 2 mM | 0.13 | 49 | 0.0027 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant K64A, D-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | D-Lyscosubstrate NADH 2 mM | 0.021 | 62 | 3.8 × 10⁻⁴ | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant K64A, D-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | L-Lyscosubstrate NADPH 2 mM | 0.34 | 13 | 0.027 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant K64A, L-Lys |
| lysine monooxygenase NbtGvariant K64A | Nocardia farcinica | L-Lyscosubstrate NADH 2 mM | 0.18 | 40 | 0.0044 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant K64A, L-Lys |
| lysine monooxygenase NbtGvariant P238R | Nocardia farcinica | NADPHO2 consumption assay | 0.6 | 0.8 | 0.79 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant P238R, NADPH |
| lysine monooxygenase NbtGvariant P238R | Nocardia farcinica | NADPHL-Lys hydroxylation assay | 0.146 | 0.7 | 0.21 | pH 7.5, 25 °C | PMID 25802330 | Open record: lysine monooxygenase NbtG variant P238R, NADPH |