CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of structural determinants of NAD(P)H selectivity and lysine binding in lysine N(6)-monooxygenase

PMID 27503802doi:10.1016/j.abb.2016.08.004Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (38 values) from this paper: L-lysine N6-monooxygenase (NbtG) from Nocardia farcinica.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.13.59

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaL-lysine—110.09pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaNADHno L-Lys (oxidase activity)1.320.67pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaNADH2 mM L-Lys; cosubstrate L-Lys (2 mM)2.42.60.94pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaNADPHoxygen consumption assay (Oxygraph)1.70.384.5pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaNADPHoxygen consumption assay (Oxygraph); 2 mM L-Lys2.40.45.95pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant E216QNocardia farcinicaNADPHfit with substrate inhibition (K_I printed); NADPH varied0.620.90.7pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant M239RNocardia farcinicaNADH0.70.088.9pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant M239RNocardia farcinicaNADPHoxygen consumption assay (Oxygraph)0.980.0910.7pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant M239RNocardia farcinicaNADPHfit with substrate inhibition (K_I printed); NADPH varied0.0130.080.16pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant R301ANocardia farcinicaL-lysine—0.50.54pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant R301ANocardia farcinicaNADHoxygen consumption assay (Oxygraph)0.542.10.255pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant R301ANocardia farcinicaNADHoxygen consumption assay (Oxygraph); 2 mM L-Lys0.81.50.545pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant R301ANocardia farcinicaNADHfit with substrate inhibition (K_I printed); NADH varied0.282.10.13pH 7.5, 25 °COpen
L-lysine N6-monooxygenase (NbtG)variant R301ANocardia farcinicaNADPH——0.026pH 7.5, 25 °COpen