Values CatLog read from this paper

Substitutions at the cofactor phosphate-binding site of a clostridial alcohol dehydrogenase lead to unexpected changes in substrate specificity

PMID 26034298doi:10.1093/protein/gzv028Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (71 values) from this paper: CaADH from Clostridium autoethanogenum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.1.1.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
CaADH	Clostridium autoethanogenum	acetoin	150	98	1.6	pH 7.5, 25 °C	Open record: CaADH, acetoin
CaADH	Clostridium autoethanogenum	acetone	55	1	55	pH 7.5, 25 °C	Open record: CaADH, acetone
CaADH	Clostridium autoethanogenum	butanone	37	1.8	21	pH 7.5, 25 °C	Open record: CaADH, butanone
CaADH	Clostridium autoethanogenum	nicotinamide adenine dinucleotide 2'-phosphate (reduced)	68	0.0017	3.9 × 10⁴	pH 7.5, 25 °C	Open record: CaADH, nicotinamide adenine dinucleotide 2'-phosphate (reduced)
CaADHvariant G198D/S199V/P201E	Clostridium autoethanogenum	acetone	39	0.6	65 calc.	pH 7.5, 25 °C	Open record: CaADH variant G198D/S199V/P201E, acetone
CaADHvariant G198D/S199V/P201E/Y218A	Clostridium autoethanogenum	acetone	2.3	14	0.16	pH 7.5, 25 °C	Open record: CaADH variant G198D/S199V/P201E/Y218A, acetone
CaADHvariant G198D/S199V/P201E/Y218A	Clostridium autoethanogenum	nicotinamide adenine dinucleotide (reduced)	12	1	12	pH 7.5, 25 °C	Open record: CaADH variant G198D/S199V/P201E/Y218A, nicotinamide adenine dinucleotide (reduced)
CaADHvariant S199A	Clostridium autoethanogenum	acetoin	230	120	1.9	pH 7.5, 25 °C	Open record: CaADH variant S199A, acetoin
CaADHvariant S199A	Clostridium autoethanogenum	acetone	60	0.9	67	pH 7.5, 25 °C	Open record: CaADH variant S199A, acetone
CaADHvariant S199A	Clostridium autoethanogenum	butanone	46	0.8	61	pH 7.5, 25 °C	Open record: CaADH variant S199A, butanone
CaADHvariant S199A	Clostridium autoethanogenum	nicotinamide adenine dinucleotide 2'-phosphate (reduced)	50	0.0056	8.9 × 10³	pH 7.5, 25 °C	Open record: CaADH variant S199A, nicotinamide adenine dinucleotide 2'-phosphate (reduced)
CaADHvariant S199C	Clostridium autoethanogenum	acetoin	41	45	0.9	pH 7.5, 25 °C	Open record: CaADH variant S199C, acetoin
CaADHvariant S199C	Clostridium autoethanogenum	acetone	36	1.2	30	pH 7.5, 25 °C	Open record: CaADH variant S199C, acetone
CaADHvariant S199C	Clostridium autoethanogenum	butanone	23	1.5	15	pH 7.5, 25 °C	Open record: CaADH variant S199C, butanone
CaADHvariant S199G	Clostridium autoethanogenum	acetoin	110	230	0.48	pH 7.5, 25 °C	Open record: CaADH variant S199G, acetoin
CaADHvariant S199G	Clostridium autoethanogenum	acetone	62	1.6	39	pH 7.5, 25 °C	Open record: CaADH variant S199G, acetone
CaADHvariant S199G	Clostridium autoethanogenum	butanone	40	2.4	17	pH 7.5, 25 °C	Open record: CaADH variant S199G, butanone
CaADHvariant S199R	Clostridium autoethanogenum	acetoin	95	34	2.8	pH 7.5, 25 °C	Open record: CaADH variant S199R, acetoin
CaADHvariant S199R	Clostridium autoethanogenum	acetone	89	1.1	81	pH 7.5, 25 °C	Open record: CaADH variant S199R, acetone
CaADHvariant S199R	Clostridium autoethanogenum	butanone	50	1.9	26	pH 7.5, 25 °C	Open record: CaADH variant S199R, butanone
CaADHvariant S199R	Clostridium autoethanogenum	nicotinamide adenine dinucleotide 2'-phosphate (reduced)	32	0.036	890	pH 7.5, 25 °C	Open record: CaADH variant S199R, nicotinamide adenine dinucleotide 2'-phosphate (reduced)
CaADHvariant S199W	Clostridium autoethanogenum	acetoin	66	190	0.35	pH 7.5, 25 °C	Open record: CaADH variant S199W, acetoin
CaADHvariant S199W	Clostridium autoethanogenum	acetone	48	2	24	pH 7.5, 25 °C	Open record: CaADH variant S199W, acetone
CaADHvariant S199W	Clostridium autoethanogenum	butanone	28	3.6	7.8	pH 7.5, 25 °C	Open record: CaADH variant S199W, butanone