CatLogenzyme kinetics catalog

EC 1.1.1.4: (R,R)-butanediol dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 60 records for EC 1.1.1.4 from 6 papers, for 4 organisms. Names in these papers: CaADH, (2R,3R)-butanediol dehydrogenase (BsBDH), (2R,3R)-2,3-butanediol dehydrogenase (BDH), primary-secondary alcohol dehydrogenase (CaADH) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 55 records for EC 1.1.1.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.1.4 in Browse.

Papers

PaperOrganismsRecords
Substitutions at the cofactor phosphate-binding site of a clostridial alcohol dehydrogenase lead to unexpected changes in substrate specificityClostridium autoethanogenum24
Protein Language Model-Guided Engineering of a 2,3-Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α-Hydroxy KetonesBacillus subtilis11
Glycerol dehydrogenase plays a dual role in glycerol metabolism and 2,3-butanediol formation in Klebsiella pneumoniaeKlebsiella pneumoniae8
Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the geneSaccharomyces cerevisiae6
New insights into two yeast BDHs from the PDH subfamily as aldehyde reductases in context of detoxification of lignocellulosic aldehyde inhibitorsSaccharomyces cerevisiae6
Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenaseClostridium autoethanogenum5

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiae(2R,3R)-butane-2,3-diol1.3 × 10³1493pH 8, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiaebutane-1,2-diol6075710.7pH 8, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiaemeso-butane-2,3-diol7706512pH 8, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiaeNAD+—0.55—pH 8, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiaeNADH—0.055—pH 7, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase (BDH)Saccharomyces cerevisiaeracemic 3-hydroxybutan-2-one ((3R)- and (3S)-acetoin)1.63 × 10³4.5362pH 7, 25 °CPMID 10938079Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh1p(B)Saccharomyces cerevisiaeacetaldehyde37.320.6557.29pH 7, 30 °CPMID 32556414Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh1p(B)Saccharomyces cerevisiaeglycolaldehyde9.42829.420.3203pH 7, 30 °CPMID 32556414Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh1p(Y)Saccharomyces cerevisiaeacetaldehyde56.574.4911.93pH 7, 30 °CPMID 32556414Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh1p(Y)Saccharomyces cerevisiaeglycolaldehyde10.015.520.6463pH 7, 30 °CPMID 32556414Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh2p(Y)Saccharomyces cerevisiaeacetaldehyde96.6220.184.546pH 7, 30 °CPMID 32556414Open
(2R,3R)-2,3-butanediol dehydrogenase Bdh2p(Y)Saccharomyces cerevisiaeglycolaldehyde9.3679.381.000pH 7, 30 °CPMID 32556414Open
(2R,3R)-butanediol dehydrogenase (BsBDH)Bacillus subtiliscis-1,2-cyclohexanediolN-terminally His6-tagged SUMO fusion (pET28a-SUMO-BsBDH)1.5935.060.045pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)Bacillus subtiliscis-1,2-cyclohexanediolHis6 tag moved to the C terminus (the paper's NC- prefix)0.1430.470.0047pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)Bacillus subtilistrans-1,2-cyclohexanediol0.7669.80.011pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280LBacillus subtiliscis-1,2-cyclohexanediol3.3312.440.268pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280LBacillus subtilistrans-1,2-cyclohexanediol0.8536.350.023pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115C/L118FBacillus subtiliscis-1,2-cyclohexanediol22.155.214.255pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118MBacillus subtilistrans-1,2-cyclohexanediol18.995.53.456pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118T/Y293FBacillus subtilistrans-1,2-cyclohexanediol1.1871.630.016pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118FBacillus subtiliscis-1,2-cyclohexanediol0.785.410.145pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50-2,5-2ClFBacillus subtiliscis-1,2-cyclohexanediol6.876.741.02pH 8.5PMID 41580984Open
(2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50W/W269HBacillus subtiliscis-1,2-cyclohexanediol0.9222.410.041pH 8.5PMID 41580984Open
CaADHClostridium autoethanogenumacetoin150981.6pH 7.5, 25 °CPMID 26034298Open
CaADHClostridium autoethanogenumacetone55155pH 7.5, 25 °CPMID 26034298Open
CaADHClostridium autoethanogenumbutanone371.821pH 7.5, 25 °CPMID 26034298Open
CaADHClostridium autoethanogenumnicotinamide adenine dinucleotide 2'-phosphate (reduced)680.00173.9 × 10⁴pH 7.5, 25 °CPMID 26034298Open
CaADHvariant G198D/S199V/P201EClostridium autoethanogenumacetone390.665 calc.pH 7.5, 25 °CPMID 26034298Open
CaADHvariant G198D/S199V/P201E/Y218AClostridium autoethanogenumacetone2.3140.16pH 7.5, 25 °CPMID 26034298Open
CaADHvariant G198D/S199V/P201E/Y218AClostridium autoethanogenumnicotinamide adenine dinucleotide (reduced)12112pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199AClostridium autoethanogenumacetoin2301201.9pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199AClostridium autoethanogenumacetone600.967pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199AClostridium autoethanogenumbutanone460.861pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199AClostridium autoethanogenumnicotinamide adenine dinucleotide 2'-phosphate (reduced)500.00568.9 × 10³pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199CClostridium autoethanogenumacetoin41450.9pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199CClostridium autoethanogenumacetone361.230pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199CClostridium autoethanogenumbutanone231.515pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199GClostridium autoethanogenumacetoin1102300.48pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199GClostridium autoethanogenumacetone621.639pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199GClostridium autoethanogenumbutanone402.417pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199RClostridium autoethanogenumacetoin95342.8pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199RClostridium autoethanogenumacetone891.181pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199RClostridium autoethanogenumbutanone501.926pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199RClostridium autoethanogenumnicotinamide adenine dinucleotide 2'-phosphate (reduced)320.036890pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199WClostridium autoethanogenumacetoin661900.35pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199WClostridium autoethanogenumacetone48224pH 7.5, 25 °CPMID 26034298Open
CaADHvariant S199WClostridium autoethanogenumbutanone283.67.8pH 7.5, 25 °CPMID 26034298Open
glycerol dehydrogenase DhaDKlebsiella pneumoniae(2R,3R)-2,3-butanediol5.8236.40.03pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase DhaDKlebsiella pneumoniae3-hydroxy-2-butanone3.6812.060.31pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase DhaDKlebsiella pneumoniaeglycerol10.5193.620.11pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase DhaDKlebsiella pneumoniaemeso-2,3-butanediol4.6737.390.13pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase GldAKlebsiella pneumoniae(2R,3R)-2,3-butanediol3.82119.30.03pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase GldAKlebsiella pneumoniae3-hydroxy-2-butanone10.384.152.51pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase GldAKlebsiella pneumoniaeglycerol18.8814.491.3pH 9, 30 °CPMID 24429283Open
glycerol dehydrogenase GldAKlebsiella pneumoniaemeso-2,3-butanediol15.334.943.11pH 9, 30 °CPMID 24429283Open
primary-secondary alcohol dehydrogenase (CaADH)Clostridium autoethanogenum(2R,3R)-butane-2,3-diol2.1650.033pH 7.5, 25 °CPMID 24657865Open
primary-secondary alcohol dehydrogenase (CaADH)Clostridium autoethanogenumAcetaldehyde935.517pH 7.5, 25 °CPMID 24657865Open
primary-secondary alcohol dehydrogenase (CaADH)Clostridium autoethanogenumAcetone51.40.686pH 7.5, 25 °CPMID 24657865Open
primary-secondary alcohol dehydrogenase (CaADH)Clostridium autoethanogenumButanone441.238pH 7.5, 25 °CPMID 24657865Open
primary-secondary alcohol dehydrogenase (CaADH)Clostridium autoethanogenumRS-acetoin145712pH 7.5, 25 °CPMID 24657865Open