Values CatLog read from this paper
Protein Language Model-Guided Engineering of a 2,3-Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α-Hydroxy Ketones
PMID 41580984doi:10.1002/advs.202509314Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: (2R,3R)-butanediol dehydrogenase (BsBDH) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (2R,3R)-butanediol dehydrogenase (BsBDH) | Bacillus subtilis | cis-1,2-cyclohexanediolN-terminally His6-tagged SUMO fusion (pET28a-SUMO-BsBDH) | 1.59 | 35.06 | 0.045 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH), cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH) | Bacillus subtilis | cis-1,2-cyclohexanediolHis6 tag moved to the C terminus (the paper's NC- prefix) | 0.14 | 30.47 | 0.0047 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH), cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH) | Bacillus subtilis | trans-1,2-cyclohexanediol | 0.76 | 69.8 | 0.011 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH), trans-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L | Bacillus subtilis | cis-1,2-cyclohexanediol | 3.33 | 12.44 | 0.268 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L, cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L | Bacillus subtilis | trans-1,2-cyclohexanediol | 0.85 | 36.35 | 0.023 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L, trans-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115C/L118F | Bacillus subtilis | cis-1,2-cyclohexanediol | 22.15 | 5.21 | 4.255 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/F115C/L118F, cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118M | Bacillus subtilis | trans-1,2-cyclohexanediol | 18.99 | 5.5 | 3.456 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118M, trans-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118T/Y293F | Bacillus subtilis | trans-1,2-cyclohexanediol | 1.18 | 71.63 | 0.016 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/F115L/L118T/Y293F, trans-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F | Bacillus subtilis | cis-1,2-cyclohexanediol | 0.78 | 5.41 | 0.145 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F, cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50-2,5-2ClF | Bacillus subtilis | cis-1,2-cyclohexanediol | 6.87 | 6.74 | 1.02 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50-2,5-2ClF, cis-1,2-cyclohexanediol |
| (2R,3R)-butanediol dehydrogenase (BsBDH)variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50W/W269H | Bacillus subtilis | cis-1,2-cyclohexanediol | 0.92 | 22.41 | 0.041 | pH 8.5 | Open record: (2R,3R)-butanediol dehydrogenase (BsBDH) variant T22V/Q112N/A230R/T258G/A260M/I280L/Y293S/L118F/F50W/W269H, cis-1,2-cyclohexanediol |