Values CatLog read from this paper
Directed evolution of the tryptophan synthase β-subunit for stand-alone function recapitulates allosteric activation
PMID 26553994doi:10.1073/pnas.1516401112Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: tryptophan synthase beta-subunit PfTrpB, tryptophan synthase PfTrpS (alpha2beta2 complex of PfTrpA and PfTrpB) from Pyrococcus furiosus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tryptophan synthase beta-subunit PfTrpB | Pyrococcus furiosus | indole | 0.31 | 0.077 | 4 | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB, indole |
| tryptophan synthase beta-subunit PfTrpB | Pyrococcus furiosus | L-serine | — | 1.2 | — | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB, L-serine |
| tryptophan synthase beta-subunit PfTrpBvariant E17G/I68V/T292S/F274S/T321A | Pyrococcus furiosus | indole | 2.2 | 0.011 | 200 | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant E17G/I68V/T292S/F274S/T321A, indole |
| tryptophan synthase beta-subunit PfTrpBvariant E17G/I68V/T292S/F274S/T321A | Pyrococcus furiosus | L-serine | — | 1.2 | — | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant E17G/I68V/T292S/F274S/T321A, L-serine |
| tryptophan synthase beta-subunit PfTrpBvariant P12L/E17G/I68V/T292S/F274S/T321A | Pyrococcus furiosus | indole | 2.9 | 0.0087 | 330 | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant P12L/E17G/I68V/T292S/F274S/T321A, indole |
| tryptophan synthase beta-subunit PfTrpBvariant P12L/E17G/I68V/T292S/F274S/T321A | Pyrococcus furiosus | L-serine | — | 0.7 | — | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant P12L/E17G/I68V/T292S/F274S/T321A, L-serine |
| tryptophan synthase beta-subunit PfTrpBvariant T292S | Pyrococcus furiosus | indole | 1.1 | 0.014 | 78 | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant T292S, indole |
| tryptophan synthase beta-subunit PfTrpBvariant T292S | Pyrococcus furiosus | L-serine | — | 0.84 | — | pH 8, 75 °C | Open record: tryptophan synthase beta-subunit PfTrpB variant T292S, L-serine |
| tryptophan synthase PfTrpS (alpha2beta2 complex of PfTrpA and PfTrpB) | Pyrococcus furiosus | indole | 1 | 0.02 | 50 | pH 8, 75 °C | Open record: tryptophan synthase PfTrpS (alpha2beta2 complex of PfTrpA and PfTrpB), indole |
| tryptophan synthase PfTrpS (alpha2beta2 complex of PfTrpA and PfTrpB) | Pyrococcus furiosus | L-serine | — | 0.6 | — | pH 8, 75 °C | Open record: tryptophan synthase PfTrpS (alpha2beta2 complex of PfTrpA and PfTrpB), L-serine |