CatLogenzyme kinetics catalog

Values CatLog read from this paper

Specific Inter-residue Interactions as Determinants of Human Monoacylglycerol Lipase Catalytic Competency: A ROLE FOR GLOBAL CONFORMATIONAL CHANGES

PMID 26555264doi:10.1074/jbc.m115.670257Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (21 values) from this paper: monoacylglycerol lipase (hMGL) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.1.1.23

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
monoacylglycerol lipase (hMGL)Homo sapiens2-arachidonoylglycerol—0.025—pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S (sol-hMGL)Homo sapiens2-arachidonoylglycerol5.7 × 10⁵0.0222.6 × 10⁷ calc.pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H103A (sol-hMGL-H103A)Homo sapiens2-arachidonoylglycerol1.4 × 10⁴0.1221.3 × 10⁶pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H272A (sol-hMGL-H272A)Homo sapiens2-arachidonoylglycerol4.5 × 10⁴0.02222 × 10⁶pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H272S (sol-hMGL-H272S)Homo sapiens2-arachidonoylglycerol1.3 × 10⁴0.03084.2 × 10⁵pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H272Y (sol-hMGL-H272Y)Homo sapiens2-arachidonoylglycerol7 × 10⁴0.03622.2 × 10⁶pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H49A (sol-hMGL-H49A)Homo sapiens2-arachidonoylglycerol1.6 × 10⁵0.03664.4pH 7.4Open
monoacylglycerol lipase (hMGL)variant L169S/L176S/H54A (sol-hMGL-H54A)Homo sapiens2-arachidonoylglycerol110.012980pH 7.4Open