CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mesaconase/Fumarase FumD in Escherichia coli O157:H7 and Promiscuity of Escherichia coli Class I Fumarases FumA and FumB

PMID 26658641doi:10.1371/journal.pone.0145098Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (36 values) from this paper: class I fumarase A (FumA), class I fumarase B (FumB), fumarase D (FumD), promiscuous mesaconase/fumarase and 1 more from Escherichia coli, Escherichia coli O157:H7.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
class I fumarase A (FumA)Escherichia coli(2S,3S)-tartrate—20.635pH 6.9, 37 °COpen
class I fumarase A (FumA)Escherichia coli(S)-Citramalate—1.0835pH 6.9, 37 °COpen
class I fumarase A (FumA)Escherichia coli(S)-Malate—0.4870pH 6.9, 37 °COpen
class I fumarase A (FumA)Escherichia coliFumarate—0.0946.6 × 10³pH 6.9, 37 °COpen
class I fumarase A (FumA)Escherichia coliMesaconate—0.22250pH 6.9, 37 °COpen
class I fumarase B (FumB)Escherichia coli(2S,3S)-tartrate—0.82.13pH 6.9, 37 °COpen
class I fumarase B (FumB)Escherichia coli(S)-Citramalate—1.0438pH 6.9, 37 °COpen
class I fumarase B (FumB)Escherichia coli(S)-Malate—0.78370pH 6.9, 37 °COpen
class I fumarase B (FumB)Escherichia coliFumarate—0.213.12 × 10³pH 6.9, 37 °COpen
class I fumarase B (FumB)Escherichia coliMesaconate—0.1580pH 6.9, 37 °COpen
class II fumarase C (FumC)Escherichia coli(2S,3S)-tartrate—6.20.065pH 6.9, 37 °COpen
class II fumarase C (FumC)Escherichia coli(S)-Malate—0.93390pH 6.9, 37 °COpen
class II fumarase C (FumC)Escherichia coliFumarate—0.283.5 × 10³pH 6.9, 37 °COpen
fumarase D (FumD), promiscuous mesaconase/fumaraseEscherichia coli O157:H7(2S,3S)-tartrate—2.60.05pH 6.9, 37 °COpen
fumarase D (FumD), promiscuous mesaconase/fumaraseEscherichia coli O157:H7(S)-Citramalate—0.92110pH 6.9, 37 °COpen
fumarase D (FumD), promiscuous mesaconase/fumaraseEscherichia coli O157:H7(S)-Malate—0.2260pH 6.9, 37 °COpen
fumarase D (FumD), promiscuous mesaconase/fumaraseEscherichia coli O157:H7Fumarate—0.9750pH 6.9, 37 °COpen
fumarase D (FumD), promiscuous mesaconase/fumaraseEscherichia coli O157:H7Mesaconate—0.152 × 10³pH 6.9, 37 °COpen