CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis

PMID 2708380doi:10.1016/s0021-9258(18)83274-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 11 records (11 values) from this paper: NADPH-cytochrome P-450 oxidoreductase from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.6.2.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADPH-cytochrome P-450 oxidoreductaseRattus norvegicuscytochrome c—0.0178—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductaseRattus norvegicuscytochrome cmembrane-bound—0.0211—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductaseRattus norvegicusNADPH—0.0064—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y140D (Asp-140)Rattus norvegicuscytochrome c—0.0191—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y140D (Asp-140)Rattus norvegicusNADPH—0.0066—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y140F (Phe-140)Rattus norvegicusNADPH—0.0078—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y140F/Y178F (Phe-140/Phe-178)Rattus norvegicuscytochrome c—0.0131—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y140F/Y178F (Phe-140/Phe-178)Rattus norvegicusNADPH—0.0114—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y178D (Asp-178)Rattus norvegicusNADPH—0.0095—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y178F (Phe-178)Rattus norvegicuscytochrome c—0.014—pH 7.7, 28 °COpen
NADPH-cytochrome P-450 oxidoreductasevariant Y178F (Phe-178)Rattus norvegicusNADPH—0.0071—pH 7.7, 28 °COpen