Values CatLog read from this paper
Modified Deacetylcephalosporin C Synthase for the Biotransformation of Semisynthetic Cephalosporins
PMID 27084018doi:10.1128/aem.00174-16Open in Browse
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CatLog read 6 records (18 values) from this paper: deacetylcephalosporin C synthase (DACS) from Streptomyces clavuligerus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| deacetylcephalosporin C synthase (DACS) | Streptomyces clavuligerus | cephalosporin G | 0.055 | 2.58 | 0.0214 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS), cephalosporin G |
| deacetylcephalosporin C synthase (DACS)variant P72L/T90A/A311V (CefFTM) | Streptomyces clavuligerus | cephalosporin G | 0.122 | 2.58 | 0.0473 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS) variant P72L/T90A/A311V (CefFTM), cephalosporin G |
| deacetylcephalosporin C synthase (DACS)variant P72L/T90A/A311V/A40V/M229I/T273A (CefFM) | Streptomyces clavuligerus | cephalosporin G | 0.326 | 4.64 | 0.07033 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS) variant P72L/T90A/A311V/A40V/M229I/T273A (CefFM), cephalosporin G |
| deacetylcephalosporin C synthase (DACS)variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L (CefFOS, expressed from the codon-optimized gene cefFOS) | Streptomyces clavuligerus | cephalosporin G | 2.302 | 9.2 | 0.2502 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS) variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L (CefFOS, expressed from the codon-optimized gene cefFOS), cephalosporin G |
| deacetylcephalosporin C synthase (DACS)variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L (CefFS) | Streptomyces clavuligerus | cephalosporin G | 1.805 | 7.24 | 0.2493 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS) variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L (CefFS), cephalosporin G |
| deacetylcephalosporin C synthase (DACS)variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L/G108D (CefFGOS) | Streptomyces clavuligerus | cephalosporin G | 1.899 | 4.41 | 0.4306 | pH 7.8, 25 °C | Open record: deacetylcephalosporin C synthase (DACS) variant P72L/T90A/A311V/A40V/M229I/T273A/V171L/R182W/F267L/G108D (CefFGOS), cephalosporin G |