Values CatLog read from this paper
Point mutation (R153H or R153C) in Escherichia coli isocitrate dehydrogenase: Biochemical characterization and functional implication
PMID 27406240doi:10.1002/jobm.201600096Open in Browse
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CatLog read 10 records (20 values) from this paper: NADP+-dependent isocitrate dehydrogenase (EcIDH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADP+-dependent isocitrate dehydrogenase (EcIDH) | Escherichia coli | DL-isocitrate | 51.8 | 0.0242 | 2.1 × 10³ | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH), DL-isocitrate |
| NADP+-dependent isocitrate dehydrogenase (EcIDH) | Escherichia coli | nicotinamide adenine dinucleotide phosphate (NADP+) | — | 0.0121 | — | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH), nicotinamide adenine dinucleotide phosphate (NADP+) |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153C | Escherichia coli | DL-isocitrate | 0.3 | 0.0318 | 9 | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153C, DL-isocitrate |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153C | Escherichia coli | nicotinamide adenine dinucleotide phosphate (NADP+) | — | 0.0176 | — | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153C, nicotinamide adenine dinucleotide phosphate (NADP+) |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153C | Escherichia coli | nicotinamide adenine dinucleotide phosphate, reduced (NADPH) | — | 7.2 × 10⁻⁴ | — | pH 7.5 | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153C, nicotinamide adenine dinucleotide phosphate, reduced (NADPH) |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153C | Escherichia coli | α-ketoglutarate | 1 | 2.2 | 0.45 | pH 7.5 | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153C, α-ketoglutarate |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153H | Escherichia coli | DL-isocitrate | 0.18 | 0.0217 | 8 | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153H, DL-isocitrate |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153H | Escherichia coli | nicotinamide adenine dinucleotide phosphate (NADP+) | — | 0.011 | — | pH 7.5, 25 °C | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153H, nicotinamide adenine dinucleotide phosphate (NADP+) |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153H | Escherichia coli | nicotinamide adenine dinucleotide phosphate, reduced (NADPH) | — | 7.1 × 10⁻⁴ | — | pH 7.5 | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153H, nicotinamide adenine dinucleotide phosphate, reduced (NADPH) |
| NADP+-dependent isocitrate dehydrogenase (EcIDH)variant R153H | Escherichia coli | α-ketoglutarate | 1 | 3.3 | 0.3 | pH 7.5 | Open record: NADP+-dependent isocitrate dehydrogenase (EcIDH) variant R153H, α-ketoglutarate |