CatLogenzyme kinetics catalog

Values CatLog read from this paper

Fumarate Production by Torulopsis glabrata: Engineering Heterologous Fumarase Expression and Improving Acid Tolerance

PMID 27711153doi:10.1371/journal.pone.0164141Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 13 records (39 values) from this paper: fumarase (RoFUM) from Rhizopus oryzae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
fumarase (RoFUM)Rhizopus oryzaemalate0.055520.5740.097pH 7.3, 30 °COpen
fumarase (RoFUM)variant D162KRhizopus oryzaemalate0.065121.0880.058pH 7.3, 30 °COpen
fumarase (RoFUM)variant D162MRhizopus oryzaemalate0.05560.8350.065pH 7.3, 30 °COpen
fumarase (RoFUM)variant D162WRhizopus oryzaemalate0.010820.2160.05pH 7.3, 30 °COpen
fumarase (RoFUM)variant H159SRhizopus oryzaemalate0.039930.7830.05pH 7.3, 30 °COpen
fumarase (RoFUM)variant H159VRhizopus oryzaemalate0.047931.2670.037pH 7.3, 30 °COpen
fumarase (RoFUM)variant H159YRhizopus oryzaemalate0.09811.2820.075pH 7.3, 30 °COpen
fumarase (RoFUM)variant N161ERhizopus oryzaemalate0.00680.1560.043pH 7.3, 30 °COpen
fumarase (RoFUM)variant N161FRhizopus oryzaemalate0.029250.9090.032pH 7.3, 30 °COpen
fumarase (RoFUM)variant N161RRhizopus oryzaemalate0.044670.7970.055pH 7.3, 30 °COpen
fumarase (RoFUM)variant P160ARhizopus oryzaemalate0.034730.2680.128pH 7.3, 30 °COpen
fumarase (RoFUM)variant P160HRhizopus oryzaemalate0.0330.5590.058pH 7.3, 30 °COpen
fumarase (RoFUM)variant P160TRhizopus oryzaemalate0.028450.350.08pH 7.3, 30 °COpen