Values CatLog read from this paper
Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity
PMID 27986535doi:10.1016/j.abb.2016.12.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (87 values) from this paper: nitroreductase A (NfsA) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitroreductase A (NfsA) | Escherichia coli | (5-aziridin-1-yl)-2,4-dinitrobenzamide | 15 | 0.23 | 65 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), (5-aziridin-1-yl)-2,4-dinitrobenzamide |
| nitroreductase A (NfsA) | Escherichia coli | 1,4-Benzoquinone | 62 | 0.011 | 5.8 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 1,4-Benzoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 1,4-Dihydroxy-9,10-anthraquinone | 0.7 | 0.007 | 100 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 1,4-Dihydroxy-9,10-anthraquinone |
| nitroreductase A (NfsA) | Escherichia coli | 1,4-Naphthoquinone | 46 | 0.033 | 1.4 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 1,4-Naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 1,8-Dihydroxy-9,10-anthraquinone | 1.6 | 0.015 | 110 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 1,8-Dihydroxy-9,10-anthraquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2,3-Dichloro-1,4-naphthoquinone | 61 | 0.022 | 2.8 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,3-Dichloro-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2,4,6-trinitrophenyl-N-methylnitraminefixed 100 µM NADPH | 85 | 0.011 | 7.9 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,4,6-trinitrophenyl-N-methylnitramine |
| nitroreductase A (NfsA) | Escherichia coli | 2,4,6-trinitrophenyl-N-methylnitramineping-pong fit (Eq. 1) with both NADPH and the oxidant varied | 110 | — | — | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,4,6-trinitrophenyl-N-methylnitramine |
| nitroreductase A (NfsA) | Escherichia coli | 2,4,6-Trinitrotoluene | 89 | 0.033 | 2.7 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,4,6-Trinitrotoluene |
| nitroreductase A (NfsA) | Escherichia coli | 2,4,6-trinitrotoluene | 135 | — | — | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,4,6-trinitrotoluene |
| nitroreductase A (NfsA) | Escherichia coli | 2,6-Dimethyl-1,4-benzoquinone | 51 | 0.016 | 3.2 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2,6-Dimethyl-1,4-benzoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2-Hydroxy-1,4-naphthoquinone | 72 | 0.0066 | 1.1 × 10⁴ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2-Hydroxy-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2-Hydroxy-3-methyl-1,4-naphthoquinone | 56 | 0.0064 | 8.8 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2-Hydroxy-3-methyl-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2-Methyl-1,4-benzoquinone | 54 | 0.008 | 6.8 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2-Methyl-1,4-benzoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 2-Methyl-1,4-naphthoquinone | 36 | 0.13 | 280 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 2-Methyl-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 5,8-Dihydroxy-1,4-naphthoquinone | 12 | 9 × 10⁻⁴ | 1.3 × 10⁴ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 5,8-Dihydroxy-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 5-Hydroxy-1,4-naphthoquinone | 22 | 0.0016 | 1.4 × 10⁴ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 5-Hydroxy-1,4-naphthoquinone |
| nitroreductase A (NfsA) | Escherichia coli | 9,10-Anthraquinone-2-sulfonic acid | 1.4 | 0.074 | 19 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 9,10-Anthraquinone-2-sulfonic acid |
| nitroreductase A (NfsA) | Escherichia coli | 9,10-Phenantrenequinone | 54 | 0.012 | 4.7 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), 9,10-Phenantrenequinone |
| nitroreductase A (NfsA) | Escherichia coli | m-Dinitrobenzene | 55 | 0.046 | 1.2 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), m-Dinitrobenzene |
| nitroreductase A (NfsA) | Escherichia coli | NADPH | — | — | 3.4 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), NADPH |
| nitroreductase A (NfsA) | Escherichia coli | Nifuroxime | 180 | 0.031 | 5.9 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), Nifuroxime |
| nitroreductase A (NfsA) | Escherichia coli | Nitrobenzene | 14 | 1.5 | 9.6 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), Nitrobenzene |
| nitroreductase A (NfsA) | Escherichia coli | Nitrofurantoin | 136 | 0.019 | 7.2 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), Nitrofurantoin |
| nitroreductase A (NfsA) | Escherichia coli | o-Dinitrobenzene | 60 | 0.018 | 3.4 × 10³ | pH 7, 25 °C | Open record: nitroreductase A (NfsA), o-Dinitrobenzene |
| nitroreductase A (NfsA) | Escherichia coli | p-Dinitrobenzene | 27 | 0.037 | 740 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), p-Dinitrobenzene |
| nitroreductase A (NfsA) | Escherichia coli | p-Nitroacetophenone | 59 | 0.39 | 150 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), p-Nitroacetophenone |
| nitroreductase A (NfsA) | Escherichia coli | p-Nitrobenzaldehyde | 30 | 0.17 | 180 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), p-Nitrobenzaldehyde |
| nitroreductase A (NfsA) | Escherichia coli | p-Nitrobenzoic acid | 64 | 2.5 | 25 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), p-Nitrobenzoic acid |
| nitroreductase A (NfsA) | Escherichia coli | Riboflavin | 4.2 | 0.016 | 260 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), Riboflavin |
| nitroreductase A (NfsA) | Escherichia coli | Tetramethyl-1,4-benzoquinone | 2.5 | 1.1 | 2.3 | pH 7, 25 °C | Open record: nitroreductase A (NfsA), Tetramethyl-1,4-benzoquinone |