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Investigating the mechanism of ADP-forming acetyl-CoA synthetase from the protozoan parasite Entamoeba histolytica

PMID 28129670doi:10.1002/1873-3468.12573Open in Browse

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CatLog read 60 records (180 values) from this paper: ADP-forming acetyl-CoA synthetase (EhACD) from Entamoeba histolytica.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.2.1.13

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Open record
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	acetate	2.9	1.3	2.3	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	acetyl-coenzyme A	2.5	0.008	207	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	adenosine 5'-diphosphate	2.9	0.39	6.8	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	adenosine 5'-triphosphate	3.2	4	0.81	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	coenzyme A	4.2	0.12	36	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674A	Entamoeba histolytica	phosphate (orthophosphate)	2.6	1.42	2	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674A, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	acetate	50	3.9	13	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	acetyl-coenzyme A	16	0.022	704	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	adenosine 5'-diphosphate	25	1.44	17	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	adenosine 5'-triphosphate	53	4.6	9.8	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	coenzyme A	44	0.24	224	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674E	Entamoeba histolytica	phosphate (orthophosphate)	25	2.8	8.9	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674E, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	acetate	1.3	0.96	1.3	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	acetyl-coenzyme A	1.3	0.016	78	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	adenosine 5'-diphosphate	1.2	0.43	2.8	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	adenosine 5'-triphosphate	1.2	2.8	0.44	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	coenzyme A	1.2	0.041	28	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant D674N	Entamoeba histolytica	phosphate (orthophosphate)	0.9	0.57	1.6	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant D674N, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	acetate	3.3	0.68	4.8	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	acetyl-coenzyme A	2.7	0.01	266	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	adenosine 5'-diphosphate	2.8	0.41	6.9	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	adenosine 5'-triphosphate	3.3	2.2	1.6	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	coenzyme A	4.2	0.07	60	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533A	Entamoeba histolytica	phosphate (orthophosphate)	2.3	0.75	3.1	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533A, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	acetate	0.18	0.31	0.59	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	acetyl-coenzyme A	1.6	0.027	60	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	adenosine 5'-diphosphate	1.7	0.6	2.9	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	adenosine 5'-triphosphate	0.25	1.2	0.21	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	coenzyme A	0.25	0.011	23	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533D	Entamoeba histolytica	phosphate (orthophosphate)	1.7	0.25	7.3	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533D, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	acetate	0.26	0.4	0.67	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	acetyl-coenzyme A	0.12	0.012	10	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	adenosine 5'-diphosphate	0.27	1.19	0.2	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	adenosine 5'-triphosphate	0.28	3.9	0.07	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	coenzyme A	0.27	0.26	1.1	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533E	Entamoeba histolytica	phosphate (orthophosphate)	0.22	0.18	1.3	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533E, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	acetate	3.4	0.84	4.1	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	acetyl-coenzyme A	2.4	0.03	79	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	adenosine 5'-diphosphate	3.3	0.97	3.4	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	adenosine 5'-triphosphate	3.7	1.7	2.4	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	coenzyme A	4.1	0.13	287	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533K	Entamoeba histolytica	phosphate (orthophosphate)	3.1	3.25	0.97	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533K, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	acetate	1.5	0.76	1.9	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	acetyl-coenzyme A	1.4	0.015	92	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	adenosine 5'-diphosphate	1.4	0.57	2.5	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	adenosine 5'-triphosphate	2	7.7	0.26	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	coenzyme A	2	0.08	26	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533N	Entamoeba histolytica	phosphate (orthophosphate)	1.5	0.94	1.7	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533N, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	acetate	1.2	0.55	2.2	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	acetyl-coenzyme A	1.7	0.019	90	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	adenosine 5'-diphosphate	2.5	1.28	1.9	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	adenosine 5'-triphosphate	1.4	2.5	116	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	coenzyme A	1.3	0.011	0.57	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533Q	Entamoeba histolytica	phosphate (orthophosphate)	1.8	0.86	2.1	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533Q, phosphate (orthophosphate)
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	acetate	5.6	1.7	3.3	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, acetate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	acetyl-coenzyme A	18	0.016	1115	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, acetyl-coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	adenosine 5'-diphosphate	20	0.64	32	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, adenosine 5'-diphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	adenosine 5'-triphosphate	6.6	2.5	2.7	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, adenosine 5'-triphosphate
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	coenzyme A	7	0.1	70	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, coenzyme A
ADP-forming acetyl-CoA synthetase (EhACD)variant H533R	Entamoeba histolytica	phosphate (orthophosphate)	14	0.15	95	Open record: ADP-forming acetyl-CoA synthetase (EhACD) variant H533R, phosphate (orthophosphate)