Values CatLog read from this paper
Characterization of new recombinant 3-ketosteroid-Δ1-dehydrogenases for the biotransformation of steroids
PMID 28634849doi:10.1007/s00253-017-8378-2Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (81 values) from this paper: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), ReKstD (3-ketosteroid-Δ1-dehydrogenase) from Mycobacterium smegmatis, Rhodococcus erythropolis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 17α-hydroxypregn-4-ene-3,20-dione | 110.4 | 0.0917 | 1.2 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 17α-hydroxypregn-4-ene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione | 13.7 | 0.0373 | 370 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | 63.5 | 0.0985 | 650 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 4-Androsten-3-one-5-ene-17-carboxylic acid | 94.1 | 0.2102 | 450 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 4-Androsten-3-one-5-ene-17-carboxylic acid |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 4-androstene-3,17-dione | 51.5 | 0.1385 | 370 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 4-androstene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 9α-hydroxy-4-androstene-3,17-dione | 58.7 | 0.0369 | 1.6 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 9α-hydroxy-4-androstene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | androst-4,9(11)-diene-3,17-dione | 10.2 | 0.2121 | 48 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), androst-4,9(11)-diene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Cortexolone | 94.2 | 0.0933 | 1 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Cortexolone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Cortisone | 52.2 | 0.0853 | 610 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Cortisone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Hydrocortisone | 60.3 | 0.0401 | 1.5 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Hydrocortisone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | hydrocortisone acetate | 77.2 | 0.1003 | 770 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), hydrocortisone acetate |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | pregna-4,9(11),16-triene-3,20-dione | 14.4 | 0.075 | 190 | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), pregna-4,9(11),16-triene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Progesterone | 127.8 | 0.0667 | 1.9 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Progesterone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Testosterone | 77.5 | 0.0707 | 1.1 × 10³ | pH 7, 30 °C | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Testosterone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 17α-hydroxypregn-4-ene-3,20-dione | 21.4 | 0.0182 | 1.2 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 17α-hydroxypregn-4-ene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione | 26.9 | 0.0178 | 1.5 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | 12.8 | 0.0193 | 660 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 4-Androsten-3-one-5-ene-17-carboxylic acid | 40.8 | 0.2462 | 170 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 4-Androsten-3-one-5-ene-17-carboxylic acid |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 4-androstene-3,17-dione | 47.5 | 0.0481 | 990 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 4-androstene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 9α-hydroxy-4-androstene-3,17-dione | 21.4 | 0.0509 | 420 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 9α-hydroxy-4-androstene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | androst-4,9(11)-diene-3,17-dione | 22.3 | 0.014 | 1.6 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), androst-4,9(11)-diene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Cortexolone | 20.6 | 0.0123 | 1.7 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Cortexolone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Cortisone | 24.6 | 0.0272 | 900 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Cortisone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Hydrocortisone | 50.2 | 0.0463 | 1.1 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Hydrocortisone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | hydrocortisone acetate | 20.3 | 0.0232 | 880 | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), hydrocortisone acetate |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Progesterone | 25.4 | 0.0202 | 1.3 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Progesterone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Testosterone | 25.8 | 0.0226 | 1.1 × 10³ | pH 7, 30 °C | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Testosterone |