EC 1.3.99.4: 3-oxosteroid 1-dehydrogenase
OxidoreductasesOpen in Browse
CatLog read 59 records for EC 1.3.99.4 from 3 papers, for 4 organisms. Names in these papers: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), ReKstD (3-ketosteroid-Δ1-dehydrogenase), 3-ketosteroid-Δ1-dehydrogenase KstD2, KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 58 records for EC 1.3.99.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.3.99.4 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Characterization of new recombinant 3-ketosteroid-Δ1-dehydrogenases for the biotransformation of steroids | Mycobacterium smegmatis, Rhodococcus erythropolis | 27 |
| 3-Keto-5alpha-steroid Delta(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol… | Mycobacterium tuberculosis, Rhodococcus erythropolis | 23 |
| Purification, characterization, and application of a high activity 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum DSM 1381 | Mycobacterium neoaurum | 9 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 16,17α-epoxyprogesterone | 1.385 × 10⁴ | 0.0305 | 4.541 × 10⁵ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 16,17α-epoxyprogesterone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 17α,21-dihydroxyprogesterone 21-acetate | 8453 | 0.01584 | 5.335 × 10⁵ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 17α,21-dihydroxyprogesterone 21-acetate |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 17α-hydroxypregn-4-ene-3,20-dione | 9930 | 0.02109 | 4.709 × 10⁵ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 17α-hydroxypregn-4-ene-3,20-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 4-androstene-3,17-dione | 9625 | 0.1434 | 6.713 × 10⁴ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 4-androstene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 9α-hydroxy-4-androstene-3,17-dione | 1863 | 0.8327 | 2.24 × 10³ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 9α-hydroxy-4-androstene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | androsta-4,9(11)-diene-3,17-dione | 6475 | 0.1733 | 3.737 × 10⁴ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, androsta-4,9(11)-diene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | canrenone | 2774 | 0.06871 | 4.037 × 10⁴ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, canrenone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | hydrocortisone | 2842 | 0.9158 | 3.1 × 10³ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, hydrocortisone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | hydrocortisone acetate | 6826 | 0.1918 | 3.558 × 10⁴ | pH 8, 40 °C | PMID 31289904 | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, hydrocortisone acetate |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 17β-hydroxy-5α-androstan-3-one | — | 0.036 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 17β-hydroxy-5α-androstan-3-one |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 23,24-Bisnor-5α-cholestan-3-one acid | — | 0.074 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 23,24-Bisnor-5α-cholestan-3-one acid |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 4-androstene-3,17-dione | — | 0.104 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 4-androstene-3,17-dione |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 4-pregnene-3,20-dione | — | 0.018 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 4-pregnene-3,20-dione |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 5α-androstane-3,17-dione | — | 0.059 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 5α-androstane-3,17-dione |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 5α-pregnane-3,20-dione | — | 0.021 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 5α-pregnane-3,20-dione |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | 9α-hydroxy-4-androstene-3,17-dione | — | 0.119 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), 9α-hydroxy-4-androstene-3,17-dione |
| KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1) | Rhodococcus erythropolis | cortisol | — | 0.557 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD1 (3-ketosteroid Δ1-dehydrogenase isoenzyme 1), cortisol |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 17β-hydroxy-5α-androstan-3-one | — | 0.006 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 17β-hydroxy-5α-androstan-3-one |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 23,24-Bisnor-5α-cholestan-3-one acid | — | 0.026 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 23,24-Bisnor-5α-cholestan-3-one acid |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 4-androstene-3,17-dione | — | 0.005 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 4-androstene-3,17-dione |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 4-pregnene-3,20-dione | — | 0.003 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 4-pregnene-3,20-dione |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 5α-androstane-3,17-dione | — | 0.013 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 5α-androstane-3,17-dione |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 5α-pregnane-3,20-dione | — | 0.007 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 5α-pregnane-3,20-dione |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | 9α-hydroxy-4-androstene-3,17-dione | — | 0.058 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), 9α-hydroxy-4-androstene-3,17-dione |
| KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2) | Rhodococcus erythropolis | cortisol | — | 0.064 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD2 (3-ketosteroid Δ1-dehydrogenase isoenzyme 2), cortisol |
| KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase) | Rhodococcus erythropolis | 17β-hydroxy-5α-androstan-3-one | — | 0.036 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase), 17β-hydroxy-5α-androstan-3-one |
| KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase) | Rhodococcus erythropolis | 4-pregnene-3,20-dione | — | 0.029 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase), 4-pregnene-3,20-dione |
| KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase) | Rhodococcus erythropolis | 5α-androstane-3,17-dione | — | 0.07 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase), 5α-androstane-3,17-dione |
| KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase) | Rhodococcus erythropolis | 5α-pregnane-3,20-dione | — | 0.014 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: KSTD3 (3-keto-5α-steroid Δ1-dehydrogenase), 5α-pregnane-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 17α-hydroxypregn-4-ene-3,20-dione | 110.4 | 0.0917 | 1.2 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 17α-hydroxypregn-4-ene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione | 13.7 | 0.0373 | 370 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | 63.5 | 0.0985 | 650 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 4-Androsten-3-one-5-ene-17-carboxylic acid | 94.1 | 0.2102 | 450 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 4-Androsten-3-one-5-ene-17-carboxylic acid |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 4-androstene-3,17-dione | 51.5 | 0.1385 | 370 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 4-androstene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | 9α-hydroxy-4-androstene-3,17-dione | 58.7 | 0.0369 | 1.6 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), 9α-hydroxy-4-androstene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | androst-4,9(11)-diene-3,17-dione | 10.2 | 0.2121 | 48 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), androst-4,9(11)-diene-3,17-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Cortexolone | 94.2 | 0.0933 | 1 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Cortexolone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Cortisone | 52.2 | 0.0853 | 610 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Cortisone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Hydrocortisone | 60.3 | 0.0401 | 1.5 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Hydrocortisone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | hydrocortisone acetate | 77.2 | 0.1003 | 770 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), hydrocortisone acetate |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | pregna-4,9(11),16-triene-3,20-dione | 14.4 | 0.075 | 190 | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), pregna-4,9(11),16-triene-3,20-dione |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Progesterone | 127.8 | 0.0667 | 1.9 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Progesterone |
| MsKstD1 (3-ketosteroid-Δ1-dehydrogenase) | Mycobacterium smegmatis | Testosterone | 77.5 | 0.0707 | 1.1 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: MsKstD1 (3-ketosteroid-Δ1-dehydrogenase), Testosterone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 17α-hydroxypregn-4-ene-3,20-dione | 21.4 | 0.0182 | 1.2 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 17α-hydroxypregn-4-ene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione | 26.9 | 0.0178 | 1.5 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | 12.8 | 0.0193 | 660 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 4-Androsten-3-one-5-ene-17-carboxylic acid | 40.8 | 0.2462 | 170 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 4-Androsten-3-one-5-ene-17-carboxylic acid |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 4-androstene-3,17-dione | 47.5 | 0.0481 | 990 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 4-androstene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | 9α-hydroxy-4-androstene-3,17-dione | 21.4 | 0.0509 | 420 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), 9α-hydroxy-4-androstene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | androst-4,9(11)-diene-3,17-dione | 22.3 | 0.014 | 1.6 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), androst-4,9(11)-diene-3,17-dione |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Cortexolone | 20.6 | 0.0123 | 1.7 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Cortexolone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Cortisone | 24.6 | 0.0272 | 900 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Cortisone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Hydrocortisone | 50.2 | 0.0463 | 1.1 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Hydrocortisone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | hydrocortisone acetate | 20.3 | 0.0232 | 880 | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), hydrocortisone acetate |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Progesterone | 25.4 | 0.0202 | 1.3 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Progesterone |
| ReKstD (3-ketosteroid-Δ1-dehydrogenase) | Rhodococcus erythropolis | Testosterone | 25.8 | 0.0226 | 1.1 × 10³ | pH 7, 30 °C | PMID 28634849 | Open record: ReKstD (3-ketosteroid-Δ1-dehydrogenase), Testosterone |
| Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase) | Mycobacterium tuberculosis | 17β-hydroxy-5α-androstan-3-one | — | 0.033 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase), 17β-hydroxy-5α-androstan-3-one |
| Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase) | Mycobacterium tuberculosis | 4-pregnene-3,20-dione | — | 0.065 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase), 4-pregnene-3,20-dione |
| Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase) | Mycobacterium tuberculosis | 5α-androstane-3,17-dione | — | 0.166 | — | pH 7.4, 30 °C | PMID 18031290 | Open record: Rv3537 (KSTD3 orthologue, 3-keto-5α-steroid Δ1-dehydrogenase), 5α-androstane-3,17-dione |