Values CatLog read from this paper
Purification, characterization, and application of a high activity 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum DSM 1381
PMID 31289904doi:10.1007/s00253-019-09988-5Open in Browse
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CatLog read 9 records (27 values) from this paper: 3-ketosteroid-Δ1-dehydrogenase KstD2 from Mycobacterium neoaurum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 16,17α-epoxyprogesterone | 1.385 × 10⁴ | 0.0305 | 4.541 × 10⁵ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 16,17α-epoxyprogesterone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 17α,21-dihydroxyprogesterone 21-acetate | 8453 | 0.01584 | 5.335 × 10⁵ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 17α,21-dihydroxyprogesterone 21-acetate |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 17α-hydroxypregn-4-ene-3,20-dione | 9930 | 0.02109 | 4.709 × 10⁵ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 17α-hydroxypregn-4-ene-3,20-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 4-androstene-3,17-dione | 9625 | 0.1434 | 6.713 × 10⁴ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 4-androstene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | 9α-hydroxy-4-androstene-3,17-dione | 1863 | 0.8327 | 2.24 × 10³ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, 9α-hydroxy-4-androstene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | androsta-4,9(11)-diene-3,17-dione | 6475 | 0.1733 | 3.737 × 10⁴ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, androsta-4,9(11)-diene-3,17-dione |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | canrenone | 2774 | 0.06871 | 4.037 × 10⁴ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, canrenone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | hydrocortisone | 2842 | 0.9158 | 3.1 × 10³ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, hydrocortisone |
| 3-ketosteroid-Δ1-dehydrogenase KstD2 | Mycobacterium neoaurum | hydrocortisone acetate | 6826 | 0.1918 | 3.558 × 10⁴ | pH 8, 40 °C | Open record: 3-ketosteroid-Δ1-dehydrogenase KstD2, hydrocortisone acetate |