Values CatLog read from this paper
Engineering an aldehyde dehydrogenase toward its substrates, 3-hydroxypropanal and NAD+, for enhancing the production of 3-hydroxypropionic acid
PMID 29214999doi:10.1038/s41598-017-15400-xOpen in Browse
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CatLog read 61 records (183 values) from this paper: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) from Azospirillum brasilense.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | 3-hydroxypropanal | 15 | 1.6 | 9 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | 3-hydroxypropanal | 15 | 1.6 | 9.4 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | 3-hydroxypropanal | 7.6 | 1.4 | 5.4 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | NAD^+ | 12 | 0.21 | 57 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | NAD^+ | 12 | 0.21 | 57 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | NAD^+ | 5.1 | 0.18 | 28 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) | Azospirillum brasilense | NADP^+ | 8.6 | 2.3 | 3.7 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase), NADP^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104 | Azospirillum brasilense | 3-hydroxypropanal | 15 | 2.2 | 7 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104 | Azospirillum brasilense | 3-hydroxypropanal | 7.5 | 1.6 | 4.7 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104 | Azospirillum brasilense | NAD^+ | 19 | 0.28 | 68 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104 | Azospirillum brasilense | NAD^+ | 7.9 | 0.23 | 34 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104-QR | Azospirillum brasilense | 3-hydroxypropanal | 6.7 | 0.43 | 16 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104-QR | Azospirillum brasilense | 3-hydroxypropanal | 3.7 | 0.4 | 9.3 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104-QR | Azospirillum brasilense | NAD^+ | 5.9 | 0.033 | 179 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104-QR | Azospirillum brasilense | NAD^+ | 3.1 | 0.031 | 100 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 104-QR | Azospirillum brasilense | NADP^+ | 4.7 | 0.7 | 6.8 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 104-QR, NADP^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106 | Azospirillum brasilense | 3-hydroxypropanal | 12 | 1.4 | 9 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106 | Azospirillum brasilense | 3-hydroxypropanal | 6.6 | 1.3 | 5.1 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106 | Azospirillum brasilense | NAD^+ | 20 | 0.27 | 74 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106 | Azospirillum brasilense | NAD^+ | 8.3 | 0.22 | 38 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106-QR | Azospirillum brasilense | 3-hydroxypropanal | 5 | 0.29 | 17 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106-QR | Azospirillum brasilense | 3-hydroxypropanal | 2.5 | 0.17 | 14 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106-QR | Azospirillum brasilense | NAD^+ | 4.8 | 0.025 | 192 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106-QR | Azospirillum brasilense | NAD^+ | 2.4 | 0.023 | 104 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 106-QR | Azospirillum brasilense | NADP^+ | 4 | 0.54 | 7.2 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 106-QR, NADP^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108 | Azospirillum brasilense | 3-hydroxypropanal | 11 | 0.78 | 14 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108 | Azospirillum brasilense | 3-hydroxypropanal | 5.9 | 0.87 | 6.8 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108 | Azospirillum brasilense | NAD^+ | 18 | 0.27 | 67 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108 | Azospirillum brasilense | NAD^+ | 7.9 | 0.26 | 30 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108-QR | Azospirillum brasilense | 3-hydroxypropanal | 6 | 0.17 | 35 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108-QR | Azospirillum brasilense | 3-hydroxypropanal | 3 | 0.14 | 21 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108-QR | Azospirillum brasilense | NAD^+ | 6.1 | 0.037 | 165 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108-QR | Azospirillum brasilense | NAD^+ | 2.9 | 0.028 | 104 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant 108-QR | Azospirillum brasilense | NADP^+ | 4.5 | 0.66 | 6.9 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant 108-QR, NADP^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant A110S | Azospirillum brasilense | 3-hydroxypropanal | 16 | 1.8 | 8.9 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant A110S, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant A110S | Azospirillum brasilense | NAD^+ | 10 | 0.2 | 50 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant A110S, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant A442P | Azospirillum brasilense | 3-hydroxypropanal | 18 | 1.8 | 10 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant A442P, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant A442P | Azospirillum brasilense | NAD^+ | 11 | 0.19 | 58 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant A442P, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273A | Azospirillum brasilense | 3-hydroxypropanal | 16 | 2 | 8 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273A, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273A | Azospirillum brasilense | NAD^+ | 12 | 0.16 | 75 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273A, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273E | Azospirillum brasilense | 3-hydroxypropanal | 14 | 1.6 | 8.8 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273E, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273E | Azospirillum brasilense | NAD^+ | 13 | 0.24 | 54 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273E, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273S | Azospirillum brasilense | 3-hydroxypropanal | 16 | 1.7 | 9.4 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273S, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant K273S | Azospirillum brasilense | NAD^+ | 11 | 0.16 | 69 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant K273S, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant N159V | Azospirillum brasilense | 3-hydroxypropanal | 11 | 6.8 | 1.6 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant N159V, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant N159V | Azospirillum brasilense | NAD^+ | 6 | 0.07 | 86 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant N159V, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant P444E | Azospirillum brasilense | 3-hydroxypropanal | 11 | 1.1 | 10 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant P444E, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant P444E | Azospirillum brasilense | NAD^+ | 10 | 0.17 | 59 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant P444E, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant P444T | Azospirillum brasilense | 3-hydroxypropanal | 18 | 1.7 | 11 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant P444T, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant P444T | Azospirillum brasilense | NAD^+ | 13 | 0.23 | 57 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant P444T, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant R281Q | Azospirillum brasilense | 3-hydroxypropanal | 13 | 1.3 | 10 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant R281Q, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant R281Q | Azospirillum brasilense | NAD^+ | 9 | 0.14 | 64 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant R281Q, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant T443D | Azospirillum brasilense | 3-hydroxypropanal | 15 | 1.2 | 13 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant T443D, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant T443D | Azospirillum brasilense | NAD^+ | 12 | 0.22 | 55 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant T443D, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant T443E | Azospirillum brasilense | 3-hydroxypropanal | 22 | 2.1 | 11 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant T443E, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant T443E | Azospirillum brasilense | NAD^+ | 16 | 0.28 | 57 | 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant T443E, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant WT-QR | Azospirillum brasilense | 3-hydroxypropanal | 6.8 | 0.55 | 12 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant WT-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant WT-QR | Azospirillum brasilense | 3-hydroxypropanal | 2.4 | 0.29 | 8.3 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant WT-QR, 3-hydroxypropanal |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant WT-QR | Azospirillum brasilense | NAD^+ | 7.9 | 0.044 | 180 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant WT-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant WT-QR | Azospirillum brasilense | NAD^+ | 3.4 | 0.041 | 83 | pH 6, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant WT-QR, NAD^+ |
| KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase)variant WT-QR | Azospirillum brasilense | NADP^+ | 4.4 | 0.81 | 5.4 | pH 8, 25 °C | Open record: KGSADH (alpha-ketoglutaric semialdehyde dehydrogenase) variant WT-QR, NADP^+ |