Values CatLog read from this paper
The evolution of substrate discrimination in macrolide antibiotic resistance enzymes
PMID 29317655doi:10.1038/s41467-017-02680-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 63 records (189 values) from this paper: MphI, MphB, MphK from Paenibacillus sp. LC231, Escherichia coli, Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MphB | Escherichia coli | Azithromycin | 0.189 | 0.0029 | 66 | pH 7.5 | Open record: MphB, Azithromycin |
| MphB | Escherichia coli | Erythromycin | 0.0412 | 0.0016 | 25 | pH 7.5 | Open record: MphB, Erythromycin |
| MphB | Escherichia coli | GTP | 0.602 | 0.0083 | 73 | pH 7.5 | Open record: MphB, GTP |
| MphB | Escherichia coli | Josamycin | 1.2 | 8.3 × 10⁻⁴ | 1.5 × 10³ | pH 7.5 | Open record: MphB, Josamycin |
| MphB | Escherichia coli | Roxithromycin | 0.025 | 0.0015 | 17 | pH 7.5 | Open record: MphB, Roxithromycin |
| MphB | Escherichia coli | Spiramycin | 0.11 | 0.002 | 55 | pH 7.5 | Open record: MphB, Spiramycin |
| MphB | Escherichia coli | Telithromycin | 0.77 | 0.0066 | 120 | pH 7.5 | Open record: MphB, Telithromycin |
| MphB | Escherichia coli | Tylosin | 0.07 | 0.0018 | 94 | pH 7.5 | Open record: MphB, Tylosin |
| MphI | Paenibacillus sp. LC231 | Azithromycin | 0.0217 | 0.056 | 0.39 | pH 7.5 | Open record: MphI, Azithromycin |
| MphI | Paenibacillus sp. LC231 | Erythromycin | 0.012 | 0.11 | 0.1 | pH 7.5 | Open record: MphI, Erythromycin |
| MphI | Paenibacillus sp. LC231 | GTP | 1.02 | 0.01 | 100 | pH 7.5 | Open record: MphI, GTP |
| MphI | Paenibacillus sp. LC231 | Josamycin | 4.7 | 0.03 | 160 | pH 7.5 | Open record: MphI, Josamycin |
| MphI | Paenibacillus sp. LC231 | Roxithromycin | 0.003 | 0.032 | 0.091 | pH 7.5 | Open record: MphI, Roxithromycin |
| MphI | Paenibacillus sp. LC231 | Spiramycin | 1.7 | 0.0081 | 210 | pH 7.5 | Open record: MphI, Spiramycin |
| MphI | Paenibacillus sp. LC231 | Telithromycin | 0.99 | 0.003 | 330 | pH 7.5 | Open record: MphI, Telithromycin |
| MphI | Paenibacillus sp. LC231 | Tylosin | 0.46 | 0.0025 | 180 | pH 7.5 | Open record: MphI, Tylosin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Azithromycin | 0.47 | 0.034 | 14 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Azithromycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Erythromycin | 0.197 | 0.046 | 4.3 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Erythromycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | GTP | 0.45 | 0.005 | 83 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, GTP |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Josamycin | 0.174 | 0.0041 | 43 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Josamycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Roxithromycin | 0.153 | 0.019 | 7.9 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Roxithromycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Spiramycin | 0.347 | 0.0042 | 82 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Spiramycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Telithromycin | 0.376 | 0.002 | 180 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Telithromycin |
| MphIvariant M40A/S111G/T166E/P211L | Paenibacillus sp. LC231 | Tylosin | 0.131 | 0.004 | 32 | pH 7.5 | Open record: MphI variant M40A/S111G/T166E/P211L, Tylosin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Azithromycin | 0.094 | 0.083 | 1.1 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Azithromycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Erythromycin | 0.02 | 0.079 | 0.26 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Erythromycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | GTP | 0.78 | 0.016 | 50 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, GTP |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Josamycin | 1.8 | 0.014 | 120 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Josamycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Roxithromycin | 0.0099 | 0.1 | 0.097 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Roxithromycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Spiramycin | 1.4 | 0.0069 | 200 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Spiramycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Telithromycin | 1.68 | 0.0055 | 310 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Telithromycin |
| MphIvariant M40A/S111R/T166E | Paenibacillus sp. LC231 | Tylosin | 0.7 | 0.0094 | 74 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E, Tylosin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Azithromycin | 0.56 | 0.032 | 18 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Azithromycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Erythromycin | 0.19 | 0.075 | 2.6 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Erythromycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | GTP | 0.71 | 0.0051 | 140 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, GTP |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Josamycin | 0.45 | 0.02 | 22 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Josamycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Roxithromycin | 0.145 | 0.046 | 3.2 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Roxithromycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Spiramycin | 1.3 | 0.015 | 87 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Spiramycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Telithromycin | 0.72 | 0.0056 | 130 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Telithromycin |
| MphIvariant M40A/S111R/T166E/P211L | Paenibacillus sp. LC231 | Tylosin | 0.32 | 0.0054 | 59 | pH 7.5 | Open record: MphI variant M40A/S111R/T166E/P211L, Tylosin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Azithromycin | 0.099 | 0.114 | 0.86 | pH 7.5 | Open record: MphI variant S111R/T166E, Azithromycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Erythromycin | 0.0202 | 0.157 | 0.128 | pH 7.5 | Open record: MphI variant S111R/T166E, Erythromycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | GTP | 0.55 | 0.013 | 42 | pH 7.5 | Open record: MphI variant S111R/T166E, GTP |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Josamycin | 3.7 | 0.034 | 110 | pH 7.5 | Open record: MphI variant S111R/T166E, Josamycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Roxithromycin | 0.0066 | 0.046 | 0.14 | pH 7.5 | Open record: MphI variant S111R/T166E, Roxithromycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Spiramycin | 1.01 | 0.01 | 100 | pH 7.5 | Open record: MphI variant S111R/T166E, Spiramycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Telithromycin | 1.32 | 0.0031 | 430 | pH 7.5 | Open record: MphI variant S111R/T166E, Telithromycin |
| MphIvariant S111R/T166E | Paenibacillus sp. LC231 | Tylosin | 0.37 | 0.012 | 30 | pH 7.5 | Open record: MphI variant S111R/T166E, Tylosin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Azithromycin | 0.049 | 0.093 | 0.53 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Azithromycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Erythromycin | 0.0114 | 0.14 | 0.079 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Erythromycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | GTP | 0.09 | 0.003 | 30 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, GTP |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Josamycin | 0.95 | 0.034 | 28 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Josamycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Roxithromycin | 0.0054 | 0.06 | 0.09 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Roxithromycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Spiramycin | 0.53 | 0.011 | 49 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Spiramycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Telithromycin | 0.154 | 0.005 | 31 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Telithromycin |
| MphIvariant S111R/T166E/P211L | Paenibacillus sp. LC231 | Tylosin | 0.21 | 0.0046 | 45 | pH 7.5 | Open record: MphI variant S111R/T166E/P211L, Tylosin |
| MphK | Bacillus subtilis | Azithromycin | 0.025 | 0.94 | 0.026 | pH 7.5 | Open record: MphK, Azithromycin |
| MphK | Bacillus subtilis | GTP | 0.0491 | 0.017 | 3 | pH 7.5 | Open record: MphK, GTP |
| MphK | Bacillus subtilis | Josamycin | 0.097 | 0.006389 | 15 | pH 7.5 | Open record: MphK, Josamycin |
| MphK | Bacillus subtilis | Roxithromycin | 0.005 | 0.1 | 0.049 | pH 7.5 | Open record: MphK, Roxithromycin |
| MphK | Bacillus subtilis | Spiramycin | 0.044 | 0.0079 | 5.7 | pH 7.5 | Open record: MphK, Spiramycin |
| MphK | Bacillus subtilis | Telithromycin | 0.056 | 0.0042 | 13 | pH 7.5 | Open record: MphK, Telithromycin |
| MphK | Bacillus subtilis | Tylosin | 0.0022 | 0.0063 | 0.35 | pH 7.5 | Open record: MphK, Tylosin |