CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structure-Guided Engineering of α-Keto Acid Decarboxylase for the Production of Higher Alcohols at Elevated Temperature

PMID 29953730doi:10.1002/cssc.201800944Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (20 values) from this paper: KdcA (branched-chain alpha-keto acid decarboxylase) from Lactococcus lactis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.1.1.72

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
KdcA (branched-chain alpha-keto acid decarboxylase)Lactococcus lactis3-methyl-2-oxobutanoic acid1455.128 calc.pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I (1M)Lactococcus lactis3-methyl-2-oxobutanoic acid1205.621 calc.pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290C/Q252N/D306G/E316R/F388Y/L434C (7M.D)Lactococcus lactis3-methyl-2-oxobutanoic acid69——pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290C/Q252N/D306G/E316R/F388Y/L434C (7M.D)Lactococcus lactis3-methyl-2-oxobutanoic acid—5.2—pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290C/Q252N/D306G/E316R/F388Y/L434C (7M.D)Lactococcus lactis3-methyl-2-oxobutanoic acid101——pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290C/Q252N/D306G/E316R/F388Y/L434C (7M.D)Lactococcus lactis3-methyl-2-oxobutanoic acid—4.2—pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M (2M)Lactococcus lactis3-methyl-2-oxobutanoic acid1189.313 calc.pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y (6M)Lactococcus lactis3-methyl-2-oxobutanoic acid14914.610.2 calc.pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C (7M.B)Lactococcus lactis3-methyl-2-oxobutanoic acid5.2——pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C (7M.B)Lactococcus lactis3-methyl-2-oxobutanoic acid—2.6—pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C (7M.B)Lactococcus lactis3-methyl-2-oxobutanoic acid8.8——pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C (7M.B)Lactococcus lactis3-methyl-2-oxobutanoic acid—2.7—pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C (7M.A)Lactococcus lactis3-methyl-2-oxobutanoic acid958.611 calc.pH 6.5, 30 °COpen
KdcA (branched-chain alpha-keto acid decarboxylase)variant V461I/A290V/Q252N/D306G/E316R/F388Y/L434C (7M.C)Lactococcus lactis3-methyl-2-oxobutanoic acid436.66.5 calc.pH 6.5, 30 °COpen