Values CatLog read from this paper
A broader active site in Pyrococcus horikoshii CoA disulfide reductase accommodates larger substrates and reveals evidence of subunit asymmetry
PMID 29988575doi:10.1002/2211-5463.12439Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (15 values) from this paper: coenzyme A-disulfide reductase (phCoADR) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| coenzyme A-disulfide reductase (phCoADR) | Pyrococcus horikoshii | coenzyme A disulfidecosubstrate NADPH | 4.4 | 0.052 | 85 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR), coenzyme A disulfide |
| coenzyme A-disulfide reductase (phCoADR) | Pyrococcus horikoshii | coenzyme A disulfidecosubstrate NADH | 5.43 | 0.0286 | 190 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR), coenzyme A disulfide |
| coenzyme A-disulfide reductase (phCoADR) | Pyrococcus horikoshii | NADH | 2.3 | 0.073 | 32 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR), NADH |
| coenzyme A-disulfide reductase (phCoADR) | Pyrococcus horikoshii | NADPH | 5.8 | — | — | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR), NADPH |
| coenzyme A-disulfide reductase (phCoADR)variant Y65A/Y66A/P67G/H367G | Pyrococcus horikoshii | coenzyme A disulfidecosubstrate NADPH | 18.8 | 0.057 | 330 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR) variant Y65A/Y66A/P67G/H367G, coenzyme A disulfide |
| coenzyme A-disulfide reductase (phCoADR)variant Y65A/Y66A/P67G/H367G | Pyrococcus horikoshii | coenzyme A disulfidecosubstrate NADH | 1.8 | 0.086 | 21 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR) variant Y65A/Y66A/P67G/H367G, coenzyme A disulfide |
| coenzyme A-disulfide reductase (phCoADR)variant Y65A/Y66A/P67G/H367G | Pyrococcus horikoshii | NADH | 8.1 | 0.075 | 110 calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR) variant Y65A/Y66A/P67G/H367G, NADH |
| coenzyme A-disulfide reductase (phCoADR)variant Y65A/Y66A/P67G/H367G | Pyrococcus horikoshii | NADPH | 21.6 | 0.0164 | 1.32 × 10³ calc. | pH 7.5, 75 °C | Open record: coenzyme A-disulfide reductase (phCoADR) variant Y65A/Y66A/P67G/H367G, NADPH |